Literature DB >> 26580231

Electron Microscopy of Living Cells During in Situ Fluorescence Microscopy.

Nalan Liv1, Daan S B van Oosten Slingeland1, Jean-Pierre Baudoin2, Pieter Kruit1, David W Piston2, Jacob P Hoogenboom1.   

Abstract

We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with in situ scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, that is, at a specific location in time, at a desired region of interest, guided by the dynamic fluorescence imaging. We show that this approach enables direct visualization, with EM resolution, of the distribution of bioconjugated quantum dots on cellular extensions during uptake and internalization.

Entities:  

Keywords:  epidermal growth factor; fluorescence microscopy; live cell imaging; quantum dots; scanning electron microscopy

Mesh：

Year:  2015        PMID： 26580231      PMCID： PMC4729641          DOI： 10.1021/acsnano.5b03970

Source DB:  PubMed          Journal:  ACS Nano        ISSN： 1936-0851            Impact factor:   15.881


  40 in total

1.  Radiation damage of water in environmental scanning electron microscopy.

Authors:  C P Royall; B L Thiel; A M Donald
Journal:  J Microsc       Date:  2001-12       Impact factor: 1.758

2.  Multicolor and electron microscopic imaging of connexin trafficking.

Authors:  Guido Gaietta; Thomas J Deerinck; Stephen R Adams; James Bouwer; Oded Tour; Dale W Laird; Gina E Sosinsky; Roger Y Tsien; Mark H Ellisman
Journal:  Science       Date:  2002-04-19       Impact factor: 47.728

3.  Second harmonic generating (SHG) nanoprobes for in vivo imaging.

Authors:  Periklis Pantazis; James Maloney; David Wu; Scott E Fraser
Journal:  Proc Natl Acad Sci U S A       Date:  2010-07-28       Impact factor: 11.205

4.  Atmospheric scanning electron microscope observes cells and tissues in open medium through silicon nitride film.

Authors:  Hidetoshi Nishiyama; Mitsuo Suga; Toshihiko Ogura; Yuusuke Maruyama; Mitsuru Koizumi; Kazuhiro Mio; Shinichi Kitamura; Chikara Sato
Journal:  J Struct Biol       Date:  2010-01-15       Impact factor: 2.867

5.  Immuno EM-OM correlative microscopy in solution by atmospheric scanning electron microscopy (ASEM).

Authors:  Yuusuke Maruyama; Tatsuhiko Ebihara; Hidetoshi Nishiyama; Mitsuo Suga; Chikara Sato
Journal:  J Struct Biol       Date:  2012-08-29       Impact factor: 2.867

6.  Correlative microscopy and electron tomography of GFP through photooxidation.

Authors:  Markus Grabenbauer; Willie J C Geerts; Julia Fernadez-Rodriguez; Andreas Hoenger; Abraham J Koster; Tommy Nilsson
Journal:  Nat Methods       Date:  2005-11       Impact factor: 28.547

7.  Radicals from "Good's" buffers.

Authors:  J K Grady; N D Chasteen; D C Harris
Journal:  Anal Biochem       Date:  1988-08-15       Impact factor: 3.365

8.  Observation of single colloidal platinum nanocrystal growth trajectories.

Authors:  Haimei Zheng; Rachel K Smith; Young-Wook Jun; Christian Kisielowski; Ulrich Dahmen; A Paul Alivisatos
Journal:  Science       Date:  2009-06-05       Impact factor: 47.728

9.  Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction.

Authors:  Diane S Lidke; Peter Nagy; Rainer Heintzmann; Donna J Arndt-Jovin; Janine N Post; Hernan E Grecco; Elizabeth A Jares-Erijman; Thomas M Jovin
Journal:  Nat Biotechnol       Date:  2004-01-04       Impact factor: 54.908

10.  Visualization of proteins in intact cells with a clonable tag for electron microscopy.

Authors:  Elia Diestra; Juan Fontana; Paul Guichard; Sergio Marco; Cristina Risco
Journal:  J Struct Biol       Date:  2008-12-10       Impact factor: 2.867
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  5 in total

1.  Linear Chains of HER2 Receptors Found in the Plasma Membrane Using Liquid-Phase Electron Microscopy.

Authors:  Kelly Parker; Patrick Trampert; Verena Tinnemann; Diana Peckys; Tim Dahmen; Niels de Jonge
Journal:  Biophys J       Date:  2018-06-18       Impact factor: 4.033

2.  Graphene Microcapsule Arrays for Combinatorial Electron Microscopy and Spectroscopy in Liquids.

Authors:  Alexander Yulaev; Hongxuan Guo; Evgheni Strelcov; Lei Chen; Ivan Vlassiouk; Andrei Kolmakov
Journal:  ACS Appl Mater Interfaces       Date:  2017-05-08       Impact factor: 9.229

Review 3.  Flagellotropic Bacteriophages: Opportunities and Challenges for Antimicrobial Applications.

Authors:  Nathaniel C Esteves; Birgit E Scharf
Journal:  Int J Mol Sci       Date:  2022-06-25       Impact factor: 6.208

4.  Bimodal endocytic probe for three-dimensional correlative light and electron microscopy.

Authors:  Job Fermie; Leanne de Jager; Helen E Foster; Tineke Veenendaal; Cecilia de Heus; Suzanne van Dijk; Corlinda Ten Brink; Viola Oorschot; Lin Yang; Wei Li; Wally H Müller; Stuart Howes; Andrew P Carter; Friedrich Förster; George Posthuma; Hans C Gerritsen; Judith Klumperman; Nalan Liv
Journal:  Cell Rep Methods       Date:  2022-05-16

Review 5.  The 2018 correlative microscopy techniques roadmap.

Authors:  Toshio Ando; Satya Prathyusha Bhamidimarri; Niklas Brending; H Colin-York; Lucy Collinson; Niels De Jonge; P J de Pablo; Elke Debroye; Christian Eggeling; Christian Franck; Marco Fritzsche; Hans Gerritsen; Ben N G Giepmans; Kay Grunewald; Johan Hofkens; Jacob P Hoogenboom; Kris P F Janssen; Rainer Kaufman; Judith Klumpermann; Nyoman Kurniawan; Jana Kusch; Nalan Liv; Viha Parekh; Diana B Peckys; Florian Rehfeldt; David C Reutens; Maarten B J Roeffaers; Tim Salditt; Iwan A T Schaap; Ulrich S Schwarz; Paul Verkade; Michael W Vogel; Richard Wagner; Mathias Winterhalter; Haifeng Yuan; Giovanni Zifarelli
Journal:  J Phys D Appl Phys       Date:  2018-08-31       Impact factor: 3.207
  5 in total

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