| Literature DB >> 26577782 |
Elio Fantini1, Giovanni Giuliano2.
Abstract
Virus-Induced Gene Silencing (VIGS) is an excellent reverse genetic tool for the study of gene function in plants, based on virus infection. In this chapter, we describe a high-throughput approach based on VIGS for the study of tomato fruit biochemistry. It comprises the selection of the sequence for silencing using bioinformatics tools, the cloning of the fragment in the Tobacco Rattle Virus (TRV), and the agroinfiltration of tomato fruits mediated by Agrobacterium tumefaciens.Entities:
Keywords: Reverse genetics; Silencing; TRV; Tomato; VIGS
Mesh：
Year: 2016 PMID： 26577782 DOI： 10.1007/978-1-4939-3115-6_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745