Deigilam C Esteves1, Valeria C Pereira2, Joyce M Souza1, Rogéria Keller2, Rebeca D Simões1, Lizziane K Winkelstroter Eller1, Marcus Vinicius P Rodrigues3. 1. Master's Program in Environment and Regional Development, University of Western São Paulo, Presidente Prudente, SP, Brazil. 2. School of Biomedical Science, University of Western São Paulo, Presidente Prudente, SP, Brazil. 3. Master's Program in Environment and Regional Development, University of Western São Paulo, Presidente Prudente, SP, Brazil; School of Biomedical Science, University of Western São Paulo, Presidente Prudente, SP, Brazil. Electronic address: marcusvincius@unoeste.br.
Abstract
BACKGROUND: The hospital environment is susceptible to bacterial contamination along with survival in fomites and surfaces, allowing dissemination of potential pathogenic strains. The present research aimed to evaluate the influence of biological fluids in bacterial viability on fomites and surfaces commonly present in nosocomial environment. METHODS: Four different fomites and surfaces (ceramic floor, cotton fabric fragments and synthetic fibers, and eggcrate foam mattress) were contaminated with potential pathogens (Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, and Klebsiella pneumoniae), then submitted to influence of biological fluids (blood, urine, artificial saliva). The viability of strains was evaluated at 24 hours after contamination and then in intervals of 7 days, by the colony-forming unit count technique. RESULTS: S aureus presented viability (>70 days) in all conditions tested, E faecalis and K pneumoniae had decreased viability over time, and E coli did not exhibit a growth relationship with surfaces or fluids. Persistence and adaptability capacity of potential pathogens in fomites and surfaces exposed to the patient are important for guidance, planning, and outlining of protocols for microorganism dissemination control and prevention in the health care environment.
BACKGROUND: The hospital environment is susceptible to bacterial contamination along with survival in fomites and surfaces, allowing dissemination of potential pathogenic strains. The present research aimed to evaluate the influence of biological fluids in bacterial viability on fomites and surfaces commonly present in nosocomial environment. METHODS: Four different fomites and surfaces (ceramic floor, cotton fabric fragments and synthetic fibers, and eggcrate foam mattress) were contaminated with potential pathogens (Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, and Klebsiella pneumoniae), then submitted to influence of biological fluids (blood, urine, artificial saliva). The viability of strains was evaluated at 24 hours after contamination and then in intervals of 7 days, by the colony-forming unit count technique. RESULTS: S aureus presented viability (>70 days) in all conditions tested, E faecalis and K pneumoniae had decreased viability over time, and E coli did not exhibit a growth relationship with surfaces or fluids. Persistence and adaptability capacity of potential pathogens in fomites and surfaces exposed to the patient are important for guidance, planning, and outlining of protocols for microorganism dissemination control and prevention in the health care environment.
Authors: Agata Jabłońska-Trypuć; Marcin Makuła; Maria Włodarczyk-Makuła; Elżbieta Wołejko; Urszula Wydro; Lluis Serra-Majem; Józefa Wiater Journal: Int J Environ Res Public Health Date: 2022-07-01 Impact factor: 4.614
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