| Literature DB >> 26575579 |
Marinella Rodolfi1, Claudia Maria Oliveira Longa2, Ilaria Pertot2, Solveig Tosi1, Elena Savino1, Maria Guglielminetti1, Elisa Altobelli1, Giuseppe Del Frate1, Anna Maria Picco1.
Abstract
Periglacial areas are one of the least studied habitats on Earth, especially in terms of their fungal communities. In this work, both molecular and culture-dependent methods have been used to analyse the microfungi in soils sampled on the front of the East Dosdè Glacier (Valtellina, Northern Italy). Although this survey revealed a community that was rich in fungal species, a distinct group of psychrophilic microfungi has not been detected. Most of the isolated microfungi were mesophiles, which are well adapted to the sensitive climatic changes that occur in this alpine environment. A discrepancy in the results that were obtained by means of the two diagnostic approaches suggests that the used molecular methods cannot entirely replace traditional culture-dependent methods, and vice versa.Entities:
Keywords: Culturable analysis; Microfungi; PCR-DGGE analysis; Periglacial areas; Soil
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Year: 2015 PMID: 26575579 DOI: 10.1002/jobm.201500392
Source DB: PubMed Journal: J Basic Microbiol ISSN: 0233-111X Impact factor: 2.281