AIM: The soluble isoform of the IFN-β receptor (sIFNAR2) can bind IFN-β and modulate its activity, although its role in autoimmune diseases remains unknown. METHODS: A recombinant human sIFNAR2 protein was cloned, expressed and purified after which we developed and validated an ELISA for its quantification in human serum. Serum sIFNAR2 were assessed in multiple sclerosis (MS) patients and healthy controls. RESULTS: The ELISA has a dynamic range of 3.9-250 ng/ml and a detection limit of 2.44 ng/ml. Serum sIFNAR2 were significantly lower in untreated-MS patients than in healthy controls. CONCLUSION: The ELISA is suitable for quantification of sIFNAR2 in serum and should facilitate the study of sIFNAR2 in neuroimmunological diseases such as MS.
AIM: The soluble isoform of the IFN-β receptor (sIFNAR2) can bind IFN-β and modulate its activity, although its role in autoimmune diseases remains unknown. METHODS: A recombinant human sIFNAR2 protein was cloned, expressed and purified after which we developed and validated an ELISA for its quantification in human serum. Serum sIFNAR2 were assessed in multiple sclerosis (MS) patients and healthy controls. RESULTS: The ELISA has a dynamic range of 3.9-250 ng/ml and a detection limit of 2.44 ng/ml. Serum sIFNAR2 were significantly lower in untreated-MSpatients than in healthy controls. CONCLUSION: The ELISA is suitable for quantification of sIFNAR2 in serum and should facilitate the study of sIFNAR2 in neuroimmunological diseases such as MS.
Authors: Pablo Aliaga-Gaspar; Isaac Hurtado-Guerrero; Nicolas Lundahl Ciano-Petersen; Patricia Urbaneja; Isabel Brichette-Mieg; Virginia Reyes; Jose Luis Rodriguez-Bada; Roberto Alvarez-Lafuente; Rafael Arroyo; Ester Quintana; Lluis Ramió-Torrentà; Ana Alonso; Laura Leyva; Oscar Fernández; Begoña Oliver-Martos Journal: Front Immunol Date: 2021-12-16 Impact factor: 7.561
Authors: Isaac Hurtado-Guerrero; Bruno Hernáez; María J Pinto-Medel; Esther Calonge; José L Rodriguez-Bada; Patricia Urbaneja; Ana Alonso; Natalia Mena-Vázquez; Pablo Aliaga; Shohreh Issazadeh-Navikas; José Pavia; Laura Leyva; José Alcamí; Antonio Alcamí; Óscar Fernández; Begoña Oliver-Martos Journal: J Clin Med Date: 2020-03-31 Impact factor: 4.241