| Literature DB >> 26563952 |
Shahanavaj Khan1,2, Anees A Ansari3, Abdul Arif Khan4, Rehan Ahmad5, Omar Al-Obaid5, Wael Al-Kattan6.
Abstract
Cobalt oxide nanoparticles (Co3O4-NPs) were synthesized using simple urea-based thermal decomposition method. Phase purity and particle size of as-synthesized nanoparticles were characterized through X-ray diffraction pattern (XRD) and transmission electron microscopy. Through XRD morphology of the Co3O4-NPs was found to be variable in size with range of 36 nm. In our present study, we explored the potential cytotoxic and antibacterial effects of Co3O4-NPs in human colorectal types of cancerous cells (HT29 and SW620) and also nine Gram-positive and Gram-negative bacteria. Co3O4-NPs showed promising anticancer activity against HT29 and SW620 cells with IC50 value of 2.26 and 394.5 μg/mL, respectively. However, no significant effect of Co3O4-NPs was observed against bacterial strains. Furthermore, a detailed study has been carried out to investigate the possible mechanism of cell death in HT29 cancer cell line through the analysis of expression level of anti-apoptotic Bcl2 and BclxL markers. Western blot analysis results suggested significant role of Co3O4-NPs exposure in cell death due to apoptosis.Entities:
Keywords: Cobalt oxide nanoparticles; Cytotoxicity; MTT assay; Western blot analysis
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Year: 2015 PMID： 26563952 DOI： 10.1007/s00775-015-1310-2
Source DB: PubMed Journal: J Biol Inorg Chem ISSN： 0949-8257 Impact factor: 3.358