Literature DB >> 26563142

Anti-miroestrol polyclonal antibodies: a comparison of immunogen preparations used to obtain desired antibody properties.

Tharita Kitisripanya1,2, Kamonthip Jutathis1,2, Chadathorn Inyai1,2, Jukrapun Komaikul1,2, Orapin Udomsin1,2, Gorawit Yusakul3, Hiroyuki Tanaka3, Waraporn Putalun4,5.   

Abstract

Immunogen quality is one important factor that contributes to desirable antibody characteristics. Highly specific antibodies against miroestrol can be used to develop a quality control immunoassay for Pueraria candollei products. In this study, we investigated how various immunogen preparations affect antibody properties. The results show that immunogen prepared using the Mannich reaction provides antibodies with higher specificity and sensitivity against miroestrol than immunogen prepared with the periodate reaction. The results suggest the Mannich reaction maintains the original structure of miroestrol and generates useful antibodies for developing immunoassays.

Entities:  

Keywords:  Immunoassay; Immunogen; Mannich reaction; Miroestrol; Pueraria candollei

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Year:  2015        PMID： 26563142     DOI： 10.1007/s11418-015-0949-x

Source DB:  PubMed          Journal:  J Nat Med        ISSN： 1340-3443            Impact factor:   2.343


  9 in total

1.  Synthesis and characterization of hapten-protein conjugates for antibody production against small molecules.

Authors:  K V Singh; Jasdeep Kaur; Grish C Varshney; Manoj Raje; C Raman Suri
Journal:  Bioconjug Chem       Date:  2004 Jan-Feb       Impact factor: 4.774

2.  Miroestrol: an oestrogen from the plant Pueraria mirifica.

Authors:  J C CAIN
Journal:  Nature       Date:  1960-12-03       Impact factor: 49.962

3.  Quantitative analysis of miroestrol and kwakhurin for standardisation of Thai miracle herb 'Kwao Keur' (Pueraria mirifica) and establishment of simple isolation procedure for highly estrogenic miroestrol and deoxymiroestrol.

Authors:  Satoko Shimokawa; Takuya Kumamoto; Tsutomu Ishikawa; Miho Takashi; Yoshihiro Higuchi; Chaiyo Chaichantipyuth; Sunee Chansakaow
Journal:  Nat Prod Res       Date:  2012-06-18       Impact factor: 2.861

4.  Cationization of protein antigens. I. Alteration of immunogenic properties.

Authors:  A Muckerheide; R J Apple; A J Pesce; J G Michael
Journal:  J Immunol       Date:  1987-02-01       Impact factor: 5.422

5.  Isoflavonoids from Pueraria mirifica and their estrogenic activity.

Authors:  S Chansakaow; T Ishikawa; K Sekine; M Okada; Y Higuchi; M Kudo; C Chaichantipyuth
Journal:  Planta Med       Date:  2000-08       Impact factor: 3.352

6.  Comparative analysis of the chemical constituents of two varieties of Pueraria candollei.

Authors:  Gorawit Yusakul; Waraporn Putalun; Orapin Udomsin; Thaweesak Juengwatanatrakul; Chaiyo Chaichantipyuth
Journal:  Fitoterapia       Date:  2010-09-18       Impact factor: 2.882

7.  Identification of deoxymiroestrol as the actual rejuvenating principle of "Kwao Keur", Pueraria mirifica. The known miroestrol may be an artifact.

Authors:  S Chansakaow; T Ishikawa; H Seki; K Sekine (née Yoshizawa); M Okada; C Chaichantipyuth
Journal:  J Nat Prod       Date:  2000-02       Impact factor: 4.050

8.  High performance enzyme-linked immunosorbent assay for determination of miroestrol, a potent phytoestrogen from Pueraria candollei.

Authors:  Gorawit Yusakul; Orapin Udomsin; Thaweesak Juengwatanatrakul; Hiroyuki Tanaka; Chaiyo Chaichantipyuth; Waraporn Putalun
Journal:  Anal Chim Acta       Date:  2013-05-04       Impact factor: 6.558

9.  Preparation and characterization of bisphenol A-cationized bovine serum albumin.

Authors:  Yi Feng; Youxiang Zhou; Qiang Zou; Jixing Wang; Fusheng Chen; Zhixian Gao
Journal:  J Immunol Methods       Date:  2008-11-18       Impact factor: 2.303
  9 in total
  3 in total

1.  Enzyme linked immunosorbent assay for total potent estrogenic miroestrol and deoxymiroestrol of Pueraria candollei, a Thai herb for menopause remedy.

Authors:  Gorawit Yusakul; Tharita Kitisripanya; Thaweesak Juengwatanatrakul; Seiichi Sakamoto; Hiroyuki Tanaka; Waraporn Putalun
Journal:  J Nat Med       Date:  2018-02-28       Impact factor: 2.343

2.  A lateral flow colloidal gold-based immunoassay for rapid detection of miroestrol in samples of White Kwao Krua, a phytoestrogen-rich plant.

Authors:  Tharita Kitisripanya; Chadathorn Inyai; Jukrapun Komaikul; Supaluk Krittanai; Thaweesak Juengwatanatrakul; Seiichi Sakamoto; Hiroyuki Tanaka; Satoshi Morimoto; Waraporn Putalun
Journal:  J Nat Med       Date:  2017-06-01       Impact factor: 2.343

Review 3.  Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites.

Authors:  Seiichi Sakamoto; Waraporn Putalun; Sornkanok Vimolmangkang; Waranyoo Phoolcharoen; Yukihiro Shoyama; Hiroyuki Tanaka; Satoshi Morimoto
Journal:  J Nat Med       Date:  2017-11-21       Impact factor: 2.343
  3 in total

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