Johan Hygum Dam1, Birgitte Brinkmann Olsen2, Christina Baun2, Poul-Flemming Høilund-Carlsen2,3, Helge Thisgaard2,3. 1. Department of Nuclear Medicine, Odense University Hospital, Sdr. Boulevard 29, 5000, Odense, Denmark. Johan.Dam@rsyd.dk. 2. Department of Nuclear Medicine, Odense University Hospital, Sdr. Boulevard 29, 5000, Odense, Denmark. 3. Institute of Clinical Research, University of Southern Denmark, Odense, Denmark.
Abstract
PURPOSE: The purpose of this study was to apply an analogue of bombesin, NOTA-AMBA, labeled with Co-55 or Ga-68, for preclinical imaging of prostate cancer. PROCEDURES: The peptide NOTA-AMBA was labeled with Ga-68 or Co-55 by microwave irradiation. Biodistribution in xenograft mice (PC3) was performed at 1, 4, and 24 h (only cobalt at 24 h) using a fixed amount of peptide. Four weeks post-inoculation, xenograft mice were positron emission tomography/X-ray computed tomography scanned after tail vein injection of [(68)Ga]NOTA-AMBA or [(55)Co]NOTA-AMBA. RESULTS: Labeling with Ga-68 and Co-55/57 was achieved in yields greater than 90 %. A radiochemical purity (RCP) of 95 and 90 % were obtained for Ga-68 and Co-55, respectively. Both radiopeptides showed high uptake in the intestines, stomach, pancreas, and in the tumor ([(68)Ga]NOTA-AMBA, 10.3 %ID/g at 1 h to 6.4 %ID/g at 4 h; [(57)Co]NOTA-AMBA, 8.2 %ID/g at 1 h to 5.3%ID/g at 24 h). Normal tissue cleared over time improving tumor-to-background ratios. CONCLUSIONS: NOTA-AMBA was labeled in high yields and RCP with Ga-68 and Co-55/57. High tumor uptake in a subcutaneous mouse prostate cancer model was observed. At 24 h, [(55/57)Co]NOTA-AMBA showed better tumor-to-organ ratios than [(68)Ga]NOTA-AMBA at both 1 and 4 h post-injection. Hence, for imaging, [(55)Co]NOTA-AMBA was found to be superior compared to [(68)Ga]NOTA-AMBA.
PURPOSE: The purpose of this study was to apply an analogue of bombesin, NOTA-AMBA, labeled with Co-55 or Ga-68, for preclinical imaging of prostate cancer. PROCEDURES: The peptide NOTA-AMBA was labeled with Ga-68 or Co-55 by microwave irradiation. Biodistribution in xenograft mice (PC3) was performed at 1, 4, and 24 h (only cobalt at 24 h) using a fixed amount of peptide. Four weeks post-inoculation, xenograft mice were positron emission tomography/X-ray computed tomography scanned after tail vein injection of [(68)Ga]NOTA-AMBA or [(55)Co]NOTA-AMBA. RESULTS: Labeling with Ga-68 and Co-55/57 was achieved in yields greater than 90 %. A radiochemical purity (RCP) of 95 and 90 % were obtained for Ga-68 and Co-55, respectively. Both radiopeptides showed high uptake in the intestines, stomach, pancreas, and in the tumor ([(68)Ga]NOTA-AMBA, 10.3 %ID/g at 1 h to 6.4 %ID/g at 4 h; [(57)Co]NOTA-AMBA, 8.2 %ID/g at 1 h to 5.3%ID/g at 24 h). Normal tissue cleared over time improving tumor-to-background ratios. CONCLUSIONS:NOTA-AMBA was labeled in high yields and RCP with Ga-68 and Co-55/57. High tumor uptake in a subcutaneous mouseprostate cancer model was observed. At 24 h, [(55/57)Co]NOTA-AMBA showed better tumor-to-organ ratios than [(68)Ga]NOTA-AMBA at both 1 and 4 h post-injection. Hence, for imaging, [(55)Co]NOTA-AMBA was found to be superior compared to [(68)Ga]NOTA-AMBA.
Authors: W A Breeman; L J Hofland; M de Jong; B F Bernard; A Srinivasan; D J Kwekkeboom; T J Visser; E P Krenning Journal: Int J Cancer Date: 1999-05-17 Impact factor: 7.396
Authors: Aldo Cagnolini; Jianqing Chen; Kimberly Ramos; Tina Marie Skedzielewski; Laura E Lantry; Adrian D Nunn; Rolf E Swenson; Karen E Linder Journal: Appl Radiat Isot Date: 2010-06-30 Impact factor: 1.513
Authors: H F Valdovinos; R Hernandez; S Graves; P A Ellison; T E Barnhart; C P Theuer; J W Engle; W Cai; R J Nickles Journal: Appl Radiat Isot Date: 2017-09-06 Impact factor: 1.513
Authors: Eduardo Aluicio-Sarduy; Paul A Ellison; Todd E Barnhart; Weibo Cai; Robert Jerry Nickles; Jonathan W Engle Journal: J Labelled Comp Radiopharm Date: 2018-03-12 Impact factor: 1.921
Authors: Annie A Suganya S; K J Kochurani; Madhumathy G Nair; Jiss Maria Louis; Santhosh Sankaran; R Rajagopal; K Santhosh Kumar; Parvin Abraham; Balagopal P G; Paul Sebastian; Thara Somananthan; Tessy Thomas Maliekal Journal: Sci Rep Date: 2016-11-09 Impact factor: 4.379
Authors: Javad Garousi; Ken G Andersson; Johan H Dam; Birgitte B Olsen; Bogdan Mitran; Anna Orlova; Jos Buijs; Stefan Ståhl; John Löfblom; Helge Thisgaard; Vladimir Tolmachev Journal: Sci Rep Date: 2017-07-20 Impact factor: 4.379
Authors: Sara S Rinne; Charles Dahlsson Leitao; Zahra Saleh-Nihad; Bogdan Mitran; Vladimir Tolmachev; Stefan Ståhl; John Löfblom; Anna Orlova Journal: Int J Mol Sci Date: 2020-03-13 Impact factor: 5.923