Literature DB >> 26560537

Robust Real-Time Reverse Transcription-PCR for Detection of Foot-and-Mouth Disease Virus Neutralizing Carryover Contamination.

Ji-Hyeon Hwang1, Yong-Keol Shin2, So-Yeon Park2, Jeesoo Kim1, Su-Mi Kim1, Byounghan Kim1, Jong-Hyeon Park1, Jong-Soo Lee3, Kwang-Nyeong Lee4.   

Abstract

During an outbreak of foot-and-mouth disease (FMD), real-time reverse transcription-PCR (rRT-PCR) is the most commonly used diagnostic method to detect viral RNA. However, while this assay is often conducted during the outbreak period, there is an inevitable risk of carryover contamination. This study shows that the carryover contamination can be prevented by the use of target-specific restriction endonuclease in that assay.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID： 26560537      PMCID： PMC4702723          DOI： 10.1128/JCM.01944-15

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

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Authors:  P A Kitchin; Z Szotyori; C Fromholc; N Almond
Journal:  Nature       Date:  1990-03-15       Impact factor: 49.962

2.  A thermostable sequence-specific endonuclease from Thermus aquaticus.

Authors:  S Sato; C A Hutchinson; J I Harris
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

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Authors:  C Terpstra
Journal:  Bull Off Int Epizoot       Date:  1972 May-Jun

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Authors:  E C Anderson; W J Doughty; J Anderson; R Paling
Journal:  J Comp Pathol       Date:  1979-10       Impact factor: 1.311

5.  The early pathogenesis of foot-and-mouth disease in pigs infected by contact: a quantitative time-course study using TaqMan RT-PCR.

Authors:  Soren Alexandersen; Martin B Oleksiewicz; Alex I Donaldson
Journal:  J Gen Virol       Date:  2001-04       Impact factor: 3.891

6.  Post-PCR sterilization: a method to control carryover contamination for the polymerase chain reaction.

Authors:  G D Cimino; K C Metchette; J W Tessman; J E Hearst; S T Isaacs
Journal:  Nucleic Acids Res       Date:  1991-01-11       Impact factor: 16.971

7.  Detection of carrier cattle and sheep persistently infected with foot-and-mouth disease virus by a rapid real-time RT-PCR assay.

Authors:  Zhidong Zhang; Soren Alexandersen
Journal:  J Virol Methods       Date:  2003-08       Impact factor: 2.014

8.  Use of a portable real-time reverse transcriptase-polymerase chain reaction assay for rapid detection of foot-and-mouth disease virus.

Authors:  Johnny D Callahan; Fred Brown; Fernando A Osorio; Jung H Sur; Ed Kramer; Gary W Long; Juan Lubroth; Stefanie J Ellis; Katina S Shoulars; Kristin L Gaffney; Daniel L Rock; William M Nelson
Journal:  J Am Vet Med Assoc       Date:  2002-06-01       Impact factor: 1.936

9.  Pathogenesis of foot-and-mouth disease: clearance of the virus from the circulation of cattle and goats during experimental viraemia.

Authors:  P Sutmoller; J W McVicar
Journal:  J Hyg (Lond)       Date:  1976-10

10.  Evaluation of automated RT-PCR to accelerate the laboratory diagnosis of foot-and-mouth disease virus.

Authors:  Scott M Reid; Sylvia S Grierson; Nigel P Ferris; Geoffrey H Hutchings; Soren Alexandersen
Journal:  J Virol Methods       Date:  2003-02       Impact factor: 2.014
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