| Literature DB >> 26560533 |
Guoqing Zhang1, Fangping Cai2, Ivette Lorenzana de Rivera3, Zhiyong Zhou1, Jing Zhang1, John Nkengasong1, Feng Gao4, Chunfu Yang5.
Abstract
A multiplex allele-specific (MAS) assay has been developed for the detection of HIV-1 subtype C drug resistance mutations (DRMs). We have optimized the MAS assay to determine subtype B DRMs in dried blood spots (DBS) collected from patients on antiretroviral therapy. The new assay accurately detected DRMs, including low-abundance mutations that were often missed by Sanger sequencing.Entities:
Mesh:
Year: 2015 PMID: 26560533 PMCID: PMC4702749 DOI: 10.1128/JCM.02833-15
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948