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Literature DB >> 2655276

Expression of the plum pox virus coat protein region in Escherichia coli.

D Mattanovich¹, G Himmler, M Laimer, E Maiss, F Regner, A Da Camara Machado, V Hanzer, R Casper, H W Katinger.

Abstract

A cDNA complementary to the 3' end of plum pox virus (PPV) RNA was sequenced. The sequence was investigated for the presumable coat protein cistron by computer-aided translation. A fragment containing the stop codon of the polyprotein gene and a putative virus-specific protease cleavage site was subcloned into an E. coli expression vector. It is shown by immunological analysis that the coat protein cistron is located within the subcloned region.

Entities: Species

Mesh：

Substances：
RNA, Viral

Year: 1989 PMID： 2655276 DOI： 10.1007/bf00315256

Source DB: PubMed Journal: Virus Genes ISSN： 0920-8569 Impact factor: 2.332

10 in total

1. Sequence determination of the capsid protein gene and flanking regions of tobacco etch virus: Evidence for synthesis and processing of a polyprotein in potyvirus genome expression.

Authors: R F Allison; J C Sorenson; M E Kelly; F B Armstrong; W G Dougherty
Journal: Proc Natl Acad Sci U S A Date: 1985-06 Impact factor: 11.205

2. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors: H C Birnboim; J Doly
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

3. The nucleotide sequence of tobacco vein mottling virus RNA.

Authors: L L Domier; K M Franklin; M Shahabuddin; G M Hellmann; J H Overmeyer; S T Hiremath; M F Siaw; G P Lomonossoff; J G Shaw; R E Rhoads
Journal: Nucleic Acids Res Date: 1986-07-11 Impact factor: 16.971

4. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.

Authors: E Y Chen; P H Seeburg
Journal: DNA Date: 1985-04

5. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

6. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene.

Authors: P P Abel; R S Nelson; B De; N Hoffmann; S G Rogers; R T Fraley; R N Beachy
Journal: Science Date: 1986-05-09 Impact factor: 47.728

7. Nucleotide sequence at the 3' terminus of pepper mottle virus genomic RNA: evidence for an alternative mode of potyvirus capsid protein gene organization.

Authors: W G Dougherty; R F Allison; T Dawn Parks; R E Johnston; M J Feild; F B Armstrong
Journal: Virology Date: 1985-10-30 Impact factor: 3.616

8. Expression of Pseudomonas fluorescens D-galactose dehydrogenase in E. coli.

Authors: P Buckel; E Zehelein
Journal: Gene Date: 1981-12 Impact factor: 3.688

9. Expression of alfalfa mosaic virus RNA 4 in transgenic plants confers virus resistance.

Authors: L S Loesch-Fries; D Merlo; T Zinnen; L Burhop; K Hill; K Krahn; N Jarvis; S Nelson; E Halk
Journal: EMBO J Date: 1987-07 Impact factor: 11.598

10. Expression of alfalfa mosaic virus coat protein gene confers cross-protection in transgenic tobacco and tomato plants.

Authors: N E Tumer; K M O'connell; R S Nelson; P R Sanders; R N Beachy; R T Fraley; D M Shah
Journal: EMBO J Date: 1987-05 Impact factor: 11.598