Shudong Wang1, Wei Qu2, Tao Li2, Keke Guo2, Wenya Liu1, Zheng Wang1, Jihua Fu2. 1. Department of Pharmaceutics, Jinling Hospital, Nanjing University School of Medicine China. 2. Department of Physiology, China Pharmaceutical University China.
Abstract
PURPOSE: To compare the pharmacodynamic effects of an anodyne spray (XQAS) containing extracts of two herbs, Cynanchum paniculatum (CP) and Illicium henryi (IH), with those of spray containing the vehicle alone, CP alone (CPS) or IH alone (IHS), when applied topically acute soft tissue injury (STI) in an animal model. METHODS: Acute closed STI was modeled by hammer blow in the hind leg muscle of rat. In the acute test, XQAS, vehicle and normal saline (NS) were applied topically with instantly and repeatedly every 2 h for 8 h after modeling. In the chronic test, XQAS, vehicle, NS, CPS and IHS were applied topically respectively with instantly and repeatedly every 8 h for 96 h after modeling. RESULTS: XQAS (150 μl/time) rapidly suppressed STI-caused muscle swelling, high contents of inflammatory mediators such as prostaglandin-E2, interleukin-lβ, nitric oxide and so on. XQAS (100 and 250 μl/time) also showed chronic effects with dose-dependent suppressions of muscle swelling, up-regulated mRNA expressions of nuclear factor-κB p65 (NF-κB p65), cyclooxygenase-2 and interleukin-lβ, high contents of inflammatory mediators, and muscle cells impairment and necrosis induced by STI, while XQAS was more effective than CPS or HIS on treating STI. CONCLUSION: XQAS can suppress STI-caused increased gene expressions of NF-κB p65 and its downstream genes which mediate biosyntheses of inflammatory mediators, resulting in suppressed swelling, inflammatory reaction and cell impairment in the injured muscle. There is a synergistic effect between CPS and IHS on curing STI.
PURPOSE: To compare the pharmacodynamic effects of an anodyne spray (XQAS) containing extracts of two herbs, Cynanchum paniculatum (CP) and Illicium henryi (IH), with those of spray containing the vehicle alone, CP alone (CPS) or IH alone (IHS), when applied topically acute soft tissue injury (STI) in an animal model. METHODS: Acute closed STI was modeled by hammer blow in the hind leg muscle of rat. In the acute test, XQAS, vehicle and normal saline (NS) were applied topically with instantly and repeatedly every 2 h for 8 h after modeling. In the chronic test, XQAS, vehicle, NS, CPS and IHS were applied topically respectively with instantly and repeatedly every 8 h for 96 h after modeling. RESULTS: XQAS (150 μl/time) rapidly suppressed STI-caused muscle swelling, high contents of inflammatory mediators such as prostaglandin-E2, interleukin-lβ, nitric oxide and so on. XQAS (100 and 250 μl/time) also showed chronic effects with dose-dependent suppressions of muscle swelling, up-regulated mRNA expressions of nuclear factor-κB p65 (NF-κB p65), cyclooxygenase-2 and interleukin-lβ, high contents of inflammatory mediators, and muscle cells impairment and necrosis induced by STI, while XQAS was more effective than CPS or HIS on treating STI. CONCLUSION: XQAS can suppress STI-caused increased gene expressions of NF-κB p65 and its downstream genes which mediate biosyntheses of inflammatory mediators, resulting in suppressed swelling, inflammatory reaction and cell impairment in the injured muscle. There is a synergistic effect between CPS and IHS on curing STI.
Authors: S H Lee; E Soyoola; P Chanmugam; S Hart; W Sun; H Zhong; S Liou; D Simmons; D Hwang Journal: J Biol Chem Date: 1992-12-25 Impact factor: 5.157
Authors: K D Schaser; B Vollmar; M D Menger; L Schewior; S N Kroppenstedt; M Raschke; A S Lübbe; N P Haas; T Mittlmeier Journal: J Orthop Res Date: 1999-09 Impact factor: 3.494