Alana F Pires1,2, Natália V F C Rodrigues3, Pedro Marcos G Soares4, Ronaldo de Albuquerque Ribeiro4, Karoline S Aragão4, Márcia M Marinho5, Mayara T L da Silva5, Benildo S Cavada5, Ana Maria S Assreuy6. 1. Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Paranjana, 1700, Itaperi, Fortaleza, CE, 60.740-000, Brazil. alana_pires@yahoo.com.br. 2. Centro de Ciências da Saúde, Unidade Via Corpvs, Centro Universitário Estácio do Ceará, Rua Eliseu Uchoa Becco, 600, Fortaleza, CE, 60.810-270, Brazil. alana_pires@yahoo.com.br. 3. Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Paranjana, 1700, Itaperi, Fortaleza, CE, 60.740-000, Brazil. 4. Departamento de Morfologia e Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Rua Delmiro de Farias, s/n, Rodolfo Teófilo, Fortaleza, CE, 60.416-030, Brazil. 5. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Av. Mister Hull, s/n, Fortaleza, CE, 60.451-970, Brazil. 6. Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Paranjana, 1700, Itaperi, Fortaleza, CE, 60.740-000, Brazil. anassreuy@gmail.com.
Abstract
OBJECTIVE AND DESIGN: This study had investigated the anti-inflammatory activity of a seed lectin (LAL) isolated from Lonchocarpus araripensis. MATERIAL/ METHODS: LAL was purified by affinity chromatography (chitin column) and ion exchange chromatography (DEAE-Sephacel). In vitro LAL was tested for hemagglutinating activity against rabbit erythrocytes. In vivo LAL was assessed for the anti-inflammatory activity via intravenous injection (i.v.) in Swiss mice (25-30 g; n = 6/group) in models of paw edema and peritonitis. STATISTICAL ANALYSIS: ANOVA (p < 0.05). RESULTS: LAL revealed two bands of 30 and 60 kDa (SDS-PAGE) and exhibited hemagglutinating activity. LAL (10 mg/kg) inhibited the paw edema (77%) and vascular permeability (26%) induced by carrageenan, and the paw edema induced by serotonin (80%), bradykinin (49%), sodium nitroprusside (83%), TNF-α (75%) and PGE2 (64%). LAL also inhibited the neutrophil migration induced by fMLP (70%) or carrageenan (69%). The intravital microscopy showed that LAL inhibited rolling (83%) and adhesion (70%) of leukocytes. LAL anti-inflammatory effect was reversed by its association with N-acetyl-glucosamine. The nine-daily treatment with LAL (10 mg/kg; i.v.) showed no toxicity. CONCLUSION: The novel N-acetyl-D-glucosamine-binding lectin isolated from L. araripensis seeds presents anti-inflammatory effect involving the lectin domain and the inhibition of 5-HT, BK, PGE2, NO, TNF-α and leukocyte rolling and adhesion.
OBJECTIVE AND DESIGN: This study had investigated the anti-inflammatory activity of a seed lectin (LAL) isolated from Lonchocarpus araripensis. MATERIAL/ METHODS: LAL was purified by affinity chromatography (chitin column) and ion exchange chromatography (DEAE-Sephacel). In vitro LAL was tested for hemagglutinating activity against rabbit erythrocytes. In vivo LAL was assessed for the anti-inflammatory activity via intravenous injection (i.v.) in Swiss mice (25-30 g; n = 6/group) in models of paw edema and peritonitis. STATISTICAL ANALYSIS: ANOVA (p < 0.05). RESULTS: LAL revealed two bands of 30 and 60 kDa (SDS-PAGE) and exhibited hemagglutinating activity. LAL (10 mg/kg) inhibited the paw edema (77%) and vascular permeability (26%) induced by carrageenan, and the paw edema induced by serotonin (80%), bradykinin (49%), sodium nitroprusside (83%), TNF-α (75%) and PGE2 (64%). LAL also inhibited the neutrophil migration induced by fMLP (70%) or carrageenan (69%). The intravital microscopy showed that LAL inhibited rolling (83%) and adhesion (70%) of leukocytes. LAL anti-inflammatory effect was reversed by its association with N-acetyl-glucosamine. The nine-daily treatment with LAL (10 mg/kg; i.v.) showed no toxicity. CONCLUSION: The novel N-acetyl-D-glucosamine-binding lectin isolated from L. araripensis seeds presents anti-inflammatory effect involving the lectin domain and the inhibition of 5-HT, BK, PGE2, NO, TNF-α and leukocyte rolling and adhesion.
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