Francisco Lucas Faustino do Nascimento1, Alana de Freitas Pires1,2, Mário Rogério Lima Mota3, Pedro Henrique Chaves Isaias3, Diego Freitas de Araujo1, Maria Gleiciane de Queiroz Martins4, Cleane Gomes Moreira4, João Batista Cajazeiras4, Benildo Sousa Cavada4, Kyria Santiago do Nascimento5,6, Ana Maria Sampaio Assreuy7. 1. Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, Fortaleza, CE, CEP 60740-000, Brazil. 2. Centro de Ciências da Saúde, Unidade Via Corpvs, Centro Universitário Estácio do Ceará, Rua Eliseu Uchoa Becco 600, Fortaleza, CE, CEP 60810-270, Brazil. 3. Departamento de Farmácia, Odontologia e Enfermagem, Universidade Federal do Ceará, Rua Alexandre Baraúna 949, Fortaleza, CE, CEP 60430-170, Brazil. 4. Departamento de Bioquímica E Biologia Molecular, Laboratório de Moléculas Biologicamente Ativas (BioMol-Lab), Universidade Federal do Ceará, Campus do Pici, s/n; Bloco 907, Fortaleza, CE, CEP 60440-970, Brazil. 5. Departamento de Bioquímica E Biologia Molecular, Laboratório de Moléculas Biologicamente Ativas (BioMol-Lab), Universidade Federal do Ceará, Campus do Pici, s/n; Bloco 907, Fortaleza, CE, CEP 60440-970, Brazil. kyriasantiago@gmail.com. 6. Department of Biochemistry and Molecular Biology, Federal University of Ceará, Mister Hull s/n Bloco 907, Lab. 1075, PO Box 6043, Fortaleza, CE, 60440-970, Brazil. kyriasantiago@gmail.com. 7. Laboratório de Fisio-Farmacologia da Inflamação (LAFFIN), Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Dr. Silas Munguba 1700, Fortaleza, CE, CEP 60740-000, Brazil. anassreuy@gmail.com.
Abstract
OBJECTIVE: To investigate the effect and mechanisms of Andira anthelmia lectin in rat models of acute inflammation. MATERIAL: AAL anti-inflammatory activity was evaluated in Wistar rat models of paw edema and peritonitis. METHODS: AAL (0.01-1 mg/kg i.v.) was injected 30 min before stimulation with carrageenan and with initial and late phase inflammatory mediators into the animals paw or peritoneum for evaluation of cell migration (optical and intravital microscopy), paw edema (plethysmometry and histopathology); hyperalgesia (analgesimetry). RESULTS: AAL inhibited leukocyte migration induced by carrageenan, mainly neutrophils to the peritoneal fluid, decreasing leukocyte adhesion. In the peritoneal fluid, AAL reduced the gene expression of TNF-α and cyclooxygenase, as well the levels of PGE2. AAL inhibited the paw edema induced by carrageenan, serotonin, histamine, TNF-α, PLA2 and PGE2, but not by L-arginine. In this model, AAL also inhibited mechanical hypernociception induced by TNF-α, PGE2, db-cAMP and capsaicin, and the activity of myeloperoxidase in the paw tissues. CONCLUSION: AAL presents anti-inflammatory effect in acute models of rat inflammation involving the participation of prostaglandins, TNF-α and lectin domain.
OBJECTIVE: To investigate the effect and mechanisms of Andira anthelmia lectin in rat models of acute inflammation. MATERIAL: AAL anti-inflammatory activity was evaluated in Wistar rat models of paw edema and peritonitis. METHODS: AAL (0.01-1 mg/kg i.v.) was injected 30 min before stimulation with carrageenan and with initial and late phase inflammatory mediators into the animals paw or peritoneum for evaluation of cell migration (optical and intravital microscopy), paw edema (plethysmometry and histopathology); hyperalgesia (analgesimetry). RESULTS: AAL inhibited leukocyte migration induced by carrageenan, mainly neutrophils to the peritoneal fluid, decreasing leukocyte adhesion. In the peritoneal fluid, AAL reduced the gene expression of TNF-α and cyclooxygenase, as well the levels of PGE2. AAL inhibited the paw edema induced by carrageenan, serotonin, histamine, TNF-α, PLA2 and PGE2, but not by L-arginine. In this model, AAL also inhibited mechanical hypernociception induced by TNF-α, PGE2, db-cAMP and capsaicin, and the activity of myeloperoxidase in the paw tissues. CONCLUSION: AAL presents anti-inflammatory effect in acute models of rat inflammation involving the participation of prostaglandins, TNF-α and lectin domain.
Authors: Kyria Santiago Nascimento; Francisco Lucas Faustino do Nascimento; Mayara Torquato Lima Silva; Camila Bezerra Nobre; Cleane Gomes Moreira; Luiz André Cavalcante Brizeno; Edson Lopes da Ponte; Ana Maria Sampaio Assreuy; Benildo Sousa Cavada Journal: J Mol Recognit Date: 2015-12-06 Impact factor: 2.137
Authors: G G Vivancos; W A Verri; T M Cunha; I R S Schivo; C A Parada; F Q Cunha; S H Ferreira Journal: Braz J Med Biol Res Date: 2004-03-03 Impact factor: 2.590
Authors: E C Landucci; E Antunes; J L Donato; R Faro; S Hyslop; S Marangoni; B Oliveira; G Cirino; G de Nucci Journal: Br J Pharmacol Date: 1995-02 Impact factor: 8.739