George Patounakis1, Eric Bergh2, Eric J Forman3,4, Xin Tao3, Agnieszka Lonczak3, Jason M Franasiak3,4, Nathan Treff3,4, Richard T Scott5,6. 1. National Institutes of Health, Bethesda, MD, USA. 2. Mount Sinai School of Medicine, New York, NY, USA. 3. Reproductive Medicine Associates of New Jersey, 140 Allen Road, Basking Ridge, Morristown, NJ, 07920, USA. 4. Rutgers Robert Wood Johnson Medical School, Piscataway, NJ, USA. 5. Reproductive Medicine Associates of New Jersey, 140 Allen Road, Basking Ridge, Morristown, NJ, 07920, USA. rscott@rmanj.com. 6. Rutgers Robert Wood Johnson Medical School, Piscataway, NJ, USA. rscott@rmanj.com.
Abstract
PURPOSE: The aim of the study is to determine if thrombophilic single nucleotide polymorphisms (SNPs) affect outcomes in fresh in vitro fertilization (IVF) cycles in a large general infertility population. METHODS: A prospective cohort analysis was performed at a university-affiliated private IVF center of female patients undergoing fresh non-donor IVF cycles. The effect of the following thrombophilic SNPs on IVF outcomes were explored: factor V (Leiden and H1299R), prothrombin (G20210A), factor XIII (V34L), β-fibrinogen (-455G → A), plasminogen activator inhibitor-1 (4G/5G), human platelet antigen-1 (a/b9L33P), and methylenetetrahydrofolate reductase (C677T and A1298C). The main outcome measures included positive pregnancy test, clinical pregnancy, embryo implantation, live birth, and pregnancy loss. RESULTS: Patients (1717) were enrolled in the study, and a total of 4169 embryos were transferred. There were no statistically significant differences in positive pregnancy test, clinical pregnancy, embryo implantation, live birth, or pregnancy loss in the analysis of 1717 patients attempting their first cycle of IVF. Receiver operator characteristics and logistic regression analyses showed that outcomes cannot be predicted by the cumulative number of thrombophilic mutations present in the patient. CONCLUSIONS: Individual and cumulative thrombophilic SNPs do not affect IVF outcomes. Therefore, initial screening for these SNPs is not indicated.
PURPOSE: The aim of the study is to determine if thrombophilic single nucleotide polymorphisms (SNPs) affect outcomes in fresh in vitro fertilization (IVF) cycles in a large general infertility population. METHODS: A prospective cohort analysis was performed at a university-affiliated private IVF center of female patients undergoing fresh non-donorIVF cycles. The effect of the following thrombophilic SNPs on IVF outcomes were explored: factor V (Leiden and H1299R), prothrombin (G20210A), factor XIII (V34L), β-fibrinogen (-455G → A), plasminogen activator inhibitor-1 (4G/5G), human platelet antigen-1 (a/b9L33P), and methylenetetrahydrofolate reductase (C677T and A1298C). The main outcome measures included positive pregnancy test, clinical pregnancy, embryo implantation, live birth, and pregnancy loss. RESULTS:Patients (1717) were enrolled in the study, and a total of 4169 embryos were transferred. There were no statistically significant differences in positive pregnancy test, clinical pregnancy, embryo implantation, live birth, or pregnancy loss in the analysis of 1717 patients attempting their first cycle of IVF. Receiver operator characteristics and logistic regression analyses showed that outcomes cannot be predicted by the cumulative number of thrombophilic mutations present in the patient. CONCLUSIONS: Individual and cumulative thrombophilic SNPs do not affect IVF outcomes. Therefore, initial screening for these SNPs is not indicated.
Authors: Marcello Di Nisio; Anne W S Rutjes; Noemi Ferrante; Gian Mario Tiboni; Franco Cuccurullo; Ettore Porreca Journal: Blood Date: 2011-06-24 Impact factor: 22.113
Authors: Foad Azem; Ariel Many; Ido Ben Ami; Israel Yovel; Ami Amit; Joseph B Lessing; Michael J Kupferminc Journal: Hum Reprod Date: 2004-02 Impact factor: 6.918