César Hernández Guerrero1, Paulina Hernández Chávez2, Noemí Martínez Castro3, Alicia Parra Carriedo4, Sandra García Del Rio5, Ana Pérez Lizaur6. 1. Departamento de Salud, Universidad Iberoamericana Ciudad de México.. cesar.hernandez@ibero.mx. 2. Departamento de Salud, Universidad Iberoamericana Ciudad de México.. pauihdz@hotmail.com. 3. Departamento de Salud, Universidad Iberoamericana Ciudad de México.. macn25@hotmail.com. 4. Departamento de Salud, Universidad Iberoamericana Ciudad de México.. alicia.parra@ibero.mx. 5. Egresada de la Maestría en Nutriología Aplicada de la Universidad Iberoamericana Ciudad de México. México.. sandra_sr@hotmail.com. 6. Departamento de Salud, Universidad Iberoamericana Ciudad de México.. anabertha.perez@ibero.mx.
Abstract
INTRODUCTION: obesity affects more than a third of Mexican population. Oxidative stress participates actively in the etiology of this phenomenon. Glutathione peroxidase-1 (GPX-1) plays a protective role against oxidative stress. The SNP Pro200Leu (rs10504050) has been reported to affect the activity of the enzyme. OBJECTIVE: to determine the frequency of rs10504050 polymorphism in women with obesity and normal weight control, asses the concentration of peripheral TBARS and evaluate the consumption of pro and antioxidants. METHODS: 104 women with obesity and 70 healthy controls (CG) were included in the study. Anthropometric, biochemical, clinical and dietary features were evaluated. GPx-1 rs10504050 was determined by PCR/RFLP method. TBARS was assayed spectrophotometrically in plasma. The subjects were stratified and compared by obesity grades and by subgroups of prediabetes and diabetes condition. Statistical analysis included ANOVA of Kruskal Wallis, Xi squared and Pearson correlation. RESULTS: for rs10504050 polymorphism there were differences (Xi2 = 6; p = 0.01) between frequency (0.61) of obese carriers (Pro/Leu plus Leu/Leu) and CG carriers (0.42), and between (Xi2 = 8; p = 0.004) morbid (IMC > 40) obesity (0.74) and CG carriers. The obese group (OB) showed a prevalence of 66% of prediabetes plus diabetes. There were no differences in frequencies of rs10504050 in OB with pre or diabetes versus CG, or versus obese participants without diabetes. TBARS concentration was greater in all the degrees of OB versus CG. CONCLUSION: GPx-1 Pro200Leu polymorphism was associated with obesity especially with morbid obesity, but not with obese participants with prediabetes or diabetes. Oxidative stress is present in all grades of obesity significantly. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.
INTRODUCTION:obesity affects more than a third of Mexican population. Oxidative stress participates actively in the etiology of this phenomenon. Glutathione peroxidase-1 (GPX-1) plays a protective role against oxidative stress. The SNP Pro200Leu (rs10504050) has been reported to affect the activity of the enzyme. OBJECTIVE: to determine the frequency of rs10504050 polymorphism in women with obesity and normal weight control, asses the concentration of peripheral TBARS and evaluate the consumption of pro and antioxidants. METHODS: 104 women with obesity and 70 healthy controls (CG) were included in the study. Anthropometric, biochemical, clinical and dietary features were evaluated. GPx-1rs10504050 was determined by PCR/RFLP method. TBARS was assayed spectrophotometrically in plasma. The subjects were stratified and compared by obesity grades and by subgroups of prediabetes and diabetes condition. Statistical analysis included ANOVA of Kruskal Wallis, Xi squared and Pearson correlation. RESULTS: for rs10504050 polymorphism there were differences (Xi2 = 6; p = 0.01) between frequency (0.61) of obese carriers (Pro/Leu plus Leu/Leu) and CG carriers (0.42), and between (Xi2 = 8; p = 0.004) morbid (IMC > 40) obesity (0.74) and CG carriers. The obese group (OB) showed a prevalence of 66% of prediabetes plus diabetes. There were no differences in frequencies of rs10504050 in OB with pre or diabetes versus CG, or versus obeseparticipants without diabetes. TBARS concentration was greater in all the degrees of OB versus CG. CONCLUSION:GPx-1 Pro200Leu polymorphism was associated with obesity especially with morbid obesity, but not with obeseparticipants with prediabetes or diabetes. Oxidative stress is present in all grades of obesity significantly. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.
Authors: Alexey A Tinkov; Olga P Ajsuvakova; Tommaso Filippini; Ji-Chang Zhou; Xin Gen Lei; Eugenia R Gatiatulina; Bernhard Michalke; Margarita G Skalnaya; Marco Vinceti; Michael Aschner; Anatoly V Skalny Journal: Biomolecules Date: 2020-04-24