Literature DB >> 26528633

Isolation and Functional Analysis of Human Neutrophils.

Douglas B Kuhns1, Debra A Long Priel1, Jessica Chu2, Kol A Zarember2.   

Abstract

This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll-Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described.
Copyright © 2015 John Wiley & Sons, Inc.

Entities:  

Keywords:  NOX2; innate immunity; leukocyte; neutrophil; phagocyte

Mesh：

Substances：

Year:  2015        PMID： 26528633      PMCID： PMC4668800          DOI： 10.1002/0471142735.im0723s111

Source DB:  PubMed          Journal:  Curr Protoc Immunol        ISSN： 1934-3671


  8 in total

1.  Editorial: will the real neutrophil please stand up?

Authors:  Kol A Zarember; Douglas B Kuhns
Journal:  J Leukoc Biol       Date:  2011-12       Impact factor: 4.962

Review 2.  Neutrophils and immunity: challenges and opportunities.

Authors:  Carl Nathan
Journal:  Nat Rev Immunol       Date:  2006-03       Impact factor: 53.106

3.  The generation of highly purified primary human neutrophils and assessment of apoptosis in response to Toll-like receptor ligands.

Authors:  Lisa C Parker; Lynne R Prince; David J Buttle; Ian Sabroe
Journal:  Methods Mol Biol       Date:  2009

Review 4.  How neutrophils kill microbes.

Authors:  Anthony W Segal
Journal:  Annu Rev Immunol       Date:  2005       Impact factor: 28.527

5.  Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysaccharide.

Authors:  C Haslett; L A Guthrie; M M Kopaniak; R B Johnston; P M Henson
Journal:  Am J Pathol       Date:  1985-04       Impact factor: 4.307

6.  Toll-like receptor (TLR)2 and TLR4 in human peripheral blood granulocytes: a critical role for monocytes in leukocyte lipopolysaccharide responses.

Authors:  Ian Sabroe; Elizabeth C Jones; Lynne R Usher; Moira K B Whyte; Steven K Dower
Journal:  J Immunol       Date:  2002-05-01       Impact factor: 5.422

7.  Selective roles for Toll-like receptor (TLR)2 and TLR4 in the regulation of neutrophil activation and life span.

Authors:  Ian Sabroe; Lynne R Prince; Elizabeth C Jones; Malcolm J Horsburgh; Simon J Foster; Stefanie N Vogel; Steven K Dower; Moira K B Whyte
Journal:  J Immunol       Date:  2003-05-15       Impact factor: 5.422

Review 8.  How human neutrophils kill and degrade microbes: an integrated view.

Authors:  William M Nauseef
Journal:  Immunol Rev       Date:  2007-10       Impact factor: 12.988
  8 in total
  38 in total

Review 1.  To NET or not to NET:current opinions and state of the science regarding the formation of neutrophil extracellular traps.

Authors:  Sebastian Boeltz; Poorya Amini; Hans-Joachim Anders; Felipe Andrade; Rostyslav Bilyy; Simon Chatfield; Iwona Cichon; Danielle M Clancy; Jyaysi Desai; Tetiana Dumych; Nishant Dwivedi; Rachael Ann Gordon; Jonas Hahn; Andrés Hidalgo; Markus H Hoffmann; Mariana J Kaplan; Jason S Knight; Elzbieta Kolaczkowska; Paul Kubes; Moritz Leppkes; Angelo A Manfredi; Seamus J Martin; Christian Maueröder; Norma Maugeri; Ioannis Mitroulis; Luis E Munoz; Daigo Nakazawa; Indira Neeli; Victor Nizet; Elmar Pieterse; Marko Z Radic; Christiane Reinwald; Konstantinos Ritis; Patrizia Rovere-Querini; Michal Santocki; Christine Schauer; Georg Schett; Mark Jay Shlomchik; Hans-Uwe Simon; Panagiotis Skendros; Darko Stojkov; Peter Vandenabeele; Tom Vanden Berghe; Johan van der Vlag; Ljubomir Vitkov; Maren von Köckritz-Blickwede; Shida Yousefi; Alexander Zarbock; Martin Herrmann
Journal:  Cell Death Differ       Date:  2019-01-08       Impact factor: 15.828

2.  Inhibition of MDSC Trafficking with SX-682, a CXCR1/2 Inhibitor, Enhances NK-Cell Immunotherapy in Head and Neck Cancer Models.

Authors:  Sarah Greene; Yvette Robbins; Wojciech K Mydlarz; Angel P Huynh; Nicole C Schmitt; Jay Friedman; Lucas A Horn; Claudia Palena; Jeffrey Schlom; Dean Y Maeda; John A Zebala; Paul E Clavijo; Clint Allen
Journal:  Clin Cancer Res       Date:  2019-12-17       Impact factor: 12.531

3.  NCF1 (p47phox)-deficient chronic granulomatous disease: comprehensive genetic and flow cytometric analysis.

Authors:  Douglas B Kuhns; Amy P Hsu; David Sun; Karen Lau; Danielle Fink; Paul Griffith; Da Wei Huang; Debra A Long Priel; Laura Mendez; Samantha Kreuzburg; Christa S Zerbe; Suk See De Ravin; Harry L Malech; Steven M Holland; Xiaolin Wu; John I Gallin
Journal:  Blood Adv       Date:  2019-01-22

4.  IsoLGs (Isolevuglandins) Drive Neutrophil Migration in Hypertension and Are Essential for the Formation of Neutrophil Extracellular Traps.

Authors:  Jaya Krishnan; Néstor de la Visitación; Elizabeth M Hennen; Venkataraman Amarnath; David G Harrison; David M Patrick
Journal:  Hypertension       Date:  2022-06-10       Impact factor: 9.897

5.  The effect of loss of O-antigen ligase on phagocytic susceptibility of motile and non-motile Pseudomonas aeruginosa.

Authors:  Sally Demirdjian; Kristin Schutz; Matthew J Wargo; Joseph S Lam; Brent Berwin
Journal:  Mol Immunol       Date:  2017-10-20       Impact factor: 4.407

6.  Iguratimod dose dependently inhibits the expression of citrullinated proteins and peptidylarginine deiminases 2 and 4 in neutrophils from rheumatoid arthritis patients.

Authors:  Bingtong Li; Ping Li; Liqi Bi
Journal:  Clin Rheumatol       Date:  2019-11-22       Impact factor: 2.980

7.  Neutrophils from hereditary hemochromatosis patients are protected from iron excess and are primed.

Authors:  Cyril Renassia; Sabine Louis; Sylvain Cuvellier; Nadia Boussetta; Jean-Christophe Deschemin; Didier Borderie; Karine Bailly; Joel Poupon; Pham My-Chan Dang; Jamel El-Benna; Sandra Manceau; François Lefrère; Sophie Vaulont; Carole Peyssonnaux
Journal:  Blood Adv       Date:  2020-08-25

8.  Methodological considerations for the high sensitivity detection of multiple myeloma measurable residual disease.

Authors:  Kah Teong Soh; Joseph D Tario; Theresa E Hahn; Jens Hillengass; Philip L McCarthy; Paul K Wallace
Journal:  Cytometry B Clin Cytom       Date:  2019-12-23       Impact factor: 3.058

9.  Lipoproteins from Staphylococcus aureus Drive Neutrophil Extracellular Trap Formation in a TLR2/1- and PAD-Dependent Manner.

Authors:  Jessica S Hook; Parth A Patel; Aidan O'Malley; Lihua Xie; Jeffrey S Kavanaugh; Alexander R Horswill; Jessica G Moreland
Journal:  J Immunol       Date:  2021-07-21       Impact factor: 5.422

10.  Features of Neutrophils From Atopic and Non-Atopic Elite Endurance Runners.

Authors:  Raquel Freitas Zambonatto; Renata Nakata Teixeira; Sarah de Oliveira Poma; Eliane Borges da Silva; Mariana Mendes de Almeida; Gerson Dos Santos Leite; Cesar Miguel Momesso Dos Santos; Heloisa Helena de Oliveira Alves; Renata Gorjão; Tania Cristina Pithon-Curi; Celso R F Carvalho; Rui Curi; Adriana Cristina Levada-Pires
Journal:  Front Immunol       Date:  2021-06-11       Impact factor: 7.561
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