Benjamin Leporq1, Simon A Lambert1,2, Maxime Ronot1,3, Imane Boucenna4, Pierre Colinart4, Francois Cauchy5, Valerie Vilgrain1,3, Valerie Paradis6, Bernard E Van Beers1,3. 1. Laboratory of Imaging Biomarkers, Center of Research on Inflammation, UMR1149 INSERM-University Paris Diderot, Sorbonne Paris Cité, Paris, France. 2. BHF Centre of Excellence, Division of Imaging Sciences and Biomedical Engineering, King's College London, King's Health Partners, St. Thomas' Hospital, London, United Kingdom. 3. Department of Radiology, Beaujon University Hospital Paris Nord, Clichy, France. 4. Matière et Systèmes Complexes, UMR 7057 CNRS-University Paris Diderot, Sorbonne Paris Cité, Paris, France. 5. Department of HPB and liver transplantation, Beaujon University Hospital Paris Nord, Clichy, France. 6. Department of Pathology, Beaujon University Hospital Paris Nord, Clichy, France.
Abstract
PURPOSE: To develop an MRI method for quantifying hepatic fat content and visceral adipose tissue fatty acid composition in mice on a 7.0T preclinical system. METHODS: MR acquisitions were performed with a multiple echo spoiled gradient echo with bipolar readout gradients. After phase correction, the number of double bounds (ndb) and the number of methylene interrupted double bounds (nmidb) were quantified with a model including eight fat components, and parametric maps of saturated, monounsaturated, and polyunsaturated fatty acids were derived. The model included a complex error map to correct for the phase errors and the amplitude modulation caused by the bipolar acquisition. Validations were performed in fat-water emulsions and vegetable oils. In vivo, the feasibility was evaluated in mice receiving a high-fat diet containing primarily saturated fatty acids and a low-fat diet containing primarily unsaturated fatty acids. RESULTS: Linear regressions showed strong agreements between ndb and nmidb quantified with MRI and the theoretical values calculated using oil compositions, as well as between the proton density and the fat fractions in the emulsions. At MRI, the mouse liver fat fraction was smaller in mice fed the low-fat diet compared with mice fed the high-fat diet. In visceral adipose tissue, saturated fatty acids were significantly higher, whereas monounsaturated and polyunsaturated fatty acids were significantly lower in mice fed the low-fat diet compared with mice fed the high-fat diet. CONCLUSION: It is feasible to simultaneously quantify hepatic fat content and visceral adipose tissue fatty acid composition with 7.0T MRI in mice. Magn Reson Med 76:510-518, 2016.
PURPOSE: To develop an MRI method for quantifying hepatic fat content and visceral adipose tissue fatty acid composition in mice on a 7.0T preclinical system. METHODS: MR acquisitions were performed with a multiple echo spoiled gradient echo with bipolar readout gradients. After phase correction, the number of double bounds (ndb) and the number of methylene interrupted double bounds (nmidb) were quantified with a model including eight fat components, and parametric maps of saturated, monounsaturated, and polyunsaturated fatty acids were derived. The model included a complex error map to correct for the phase errors and the amplitude modulation caused by the bipolar acquisition. Validations were performed in fat-water emulsions and vegetable oils. In vivo, the feasibility was evaluated in mice receiving a high-fat diet containing primarily saturated fatty acids and a low-fat diet containing primarily unsaturated fatty acids. RESULTS: Linear regressions showed strong agreements between ndb and nmidb quantified with MRI and the theoretical values calculated using oil compositions, as well as between the proton density and the fat fractions in the emulsions. At MRI, the mouse liver fat fraction was smaller in mice fed the low-fat diet compared with mice fed the high-fat diet. In visceral adipose tissue, saturated fatty acids were significantly higher, whereas monounsaturated andpolyunsaturated fatty acids were significantly lower in mice fed the low-fat diet compared with mice fed the high-fat diet. CONCLUSION: It is feasible to simultaneously quantify hepatic fat content and visceral adipose tissue fatty acid composition with 7.0T MRI in mice. Magn Reson Med 76:510-518, 2016.
Authors: Kerensa M Beekman; Martine Regenboog; Aart J Nederveen; Nathalie Bravenboer; Martin den Heijer; Peter H Bisschop; Carla E Hollak; Erik M Akkerman; Mario Maas Journal: Front Endocrinol (Lausanne) Date: 2022-04-27 Impact factor: 6.055
Authors: Dianning He; Devkumar Mustafi; Xiaobing Fan; Sully Fernandez; Erica Markiewicz; Marta Zamora; Jeffrey Mueller; Joseph R Sachleben; Matthew J Brady; Suzanne D Conzen; Gregory S Karczmar Journal: PLoS One Date: 2018-01-11 Impact factor: 3.240