Prachi Shivpuje1, Renuka Ammanangi2, Kishore Bhat3, Sandeep Katti4. 1. Postgraduate Student, Department of Oral Medicine and Radiology, Maratha Mandal's NG Halgekar Institute of Dental Sciences and Research Centre, Belgaum-590010, Karnataka, India, Phone: 08971767274, e-mail: dr.prachi.katti@gmail.com. 2. Department of Oral Medicine and Radiology, Maratha Mandal's NG Halgekar Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India. 3. Department of Microbiology, Maratha Mandal's NG Halgekar Institute of Dental Sciences and Research Centre, Belgaum Karnataka, India. 4. Department of Periodontics, Maratha Mandal's Dental College Belgaum, Karnataka, India.
Abstract
BACKGROUND: Cancer till today remains the leading cause of death in both developed and developing countries. Plants have been beacon of therapeutic sources for curing diseases from times immemorial. Hence, the present study aimed at evaluating the antiproliferative activity of extract of Ocimum sanctum leaves on oral cancer cell line. OBJECTIVES: To evaluate the antiproliferative effect and to analyze dose dependent cytotoxic activity of aqueous extract of O. sanctum leaves on KB mouth cell line. To compare the effectiveness among different variety of O. sanctum. MATERIALS AND METHODS: KB cells (Mouth Epidermal Carcinoma Cells) were used for the present study. Aqueous and dry extract of O. sanctum with both dark (Krishna Tulsi) and light (Rama Tulsi) leaves were prepared in the institution. The antiproliferative and cytotoxic activity on KB cell line was evaluated by MTT assay. Statistical analysis with Mann-Whitney U-test and Wilcoxon matched pairs test was carried out. RESULTS: The aqueous extract of O. sanctum of both the leaves exhibited significant cytotoxic effect against oral cancer cell line. CONCLUSION: Aqueous extract of O. sanctum leaves was effective as an antiproliferative agent which caused apoptosis in oral cancer cell line. CLINICAL SIGNIFICANCE: Ocimum sanctum herb which is abundantly grown in India can be used for its anticancer properties for treating oral cancer. This will not only be cost-effective but will also have less or no side effects.
BACKGROUND:Cancer till today remains the leading cause of death in both developed and developing countries. Plants have been beacon of therapeutic sources for curing diseases from times immemorial. Hence, the present study aimed at evaluating the antiproliferative activity of extract of Ocimum sanctum leaves on oral cancer cell line. OBJECTIVES: To evaluate the antiproliferative effect and to analyze dose dependent cytotoxic activity of aqueous extract of O. sanctum leaves on KB mouth cell line. To compare the effectiveness among different variety of O. sanctum. MATERIALS AND METHODS: KB cells (Mouth Epidermal Carcinoma Cells) were used for the present study. Aqueous and dry extract of O. sanctum with both dark (Krishna Tulsi) and light (Rama Tulsi) leaves were prepared in the institution. The antiproliferative and cytotoxic activity on KB cell line was evaluated by MTT assay. Statistical analysis with Mann-Whitney U-test and Wilcoxon matched pairs test was carried out. RESULTS: The aqueous extract of O. sanctum of both the leaves exhibited significant cytotoxic effect against oral cancer cell line. CONCLUSION: Aqueous extract of O. sanctum leaves was effective as an antiproliferative agent which caused apoptosis in oral cancer cell line. CLINICAL SIGNIFICANCE: Ocimum sanctum herb which is abundantly grown in India can be used for its anticancer properties for treating oral cancer. This will not only be cost-effective but will also have less or no side effects.