Literature DB >> 26519169

Preprocessing and Analysis of LC-MS-Based Proteomic Data.

Tsung-Heng Tsai1,2, Minkun Wang3,4, Habtom W Ressom3.   

Abstract

Liquid chromatography coupled with mass spectrometry (LC-MS) has been widely used for profiling protein expression levels. This chapter is focused on LC-MS data preprocessing, which is a crucial step in the analysis of LC-MS based proteomics. We provide a high-level overview, highlight associated challenges, and present a step-by-step example for analysis of data from LC-MS based untargeted proteomic study. Furthermore, key procedures and relevant issues with the subsequent analysis by multiple reaction monitoring (MRM) are discussed.

Entities:  

Keywords:  Data preprocessing; Label-free; Liquid chromatography-mass spectrometry (LC-MS); Multiple reaction monitoring (MRM ); Proteomics

Mesh：

Substances：

Year:  2016        PMID： 26519169      PMCID： PMC4838194          DOI： 10.1007/978-1-4939-3106-4_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


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