Literature DB >> 26514871

Radiometric assay of ghrelin hydrolase activity and 3H-ghrelin distribution into mouse tissues.

Vicky Ping Chen1, Yang Gao2, Liyi Geng3, Stephen Brimijoin4.   

Abstract

A high-throughput radiometric assay was developed to characterize enzymatic hydrolysis of ghrelin and to track the peptide's fate in vivo. The assay is based on solvent partitioning of [(3)H]-octanoic acid liberated from [(3)H]-octanoyl ghrelin during enzymatic hydrolysis. This simple and cost-effective method facilitates kinetic analysis of ghrelin hydrolase activity of native and mutated butyrylcholinesterases or carboxylesterases from multiple species. In addition, the assay's high sensitivity facilitates ready evaluation of ghrelin's pharmacokinetics and tissue distribution in mice after i.v. bolus administration of radiolabeled peptide.
Copyright © 2015 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Butyrylcholinesterase; Di-isopropyl fluorophosphates (PubChem CID: 5936); Enzyme kinetics; Ghrelin; Human desacyl-ghrelin (PubChem CID: 71728433); Human ghrelin (PubChem CID: 91668172); Pharmacokinetics; Radiometric assay; Toluene (PubChem CID: 1140); iso-OMPA (PubChem CID: 5420)

Mesh：

Substances：

Year:  2015        PMID： 26514871      PMCID： PMC4669970          DOI： 10.1016/j.bcp.2015.10.016

Source DB:  PubMed          Journal:  Biochem Pharmacol        ISSN： 0006-2952            Impact factor:   5.858


  39 in total

1.  Pharmacokinetics, safety, and endocrine and appetite effects of ghrelin administration in young healthy subjects.

Authors:  Takashi Akamizu; Kazuhiko Takaya; Taiga Irako; Hiroshi Hosoda; Satoshi Teramukai; Akiko Matsuyama; Harue Tada; Kazumi Miura; Akira Shimizu; Masanori Fukushima; Masayuki Yokode; Koichi Tanaka; Kenji Kangawa
Journal:  Eur J Endocrinol       Date:  2004-04       Impact factor: 6.664

2.  Ghrelin induces adiposity in rodents.

Authors:  M Tschöp; D L Smiley; M L Heiman
Journal:  Nature       Date:  2000-10-19       Impact factor: 49.962

3.  Ghrelin strongly stimulates growth hormone release in humans.

Authors:  K Takaya; H Ariyasu; N Kanamoto; H Iwakura; A Yoshimoto; M Harada; K Mori; Y Komatsu; T Usui; A Shimatsu; Y Ogawa; K Hosoda; T Akamizu; M Kojima; K Kangawa; K Nakao
Journal:  J Clin Endocrinol Metab       Date:  2000-12       Impact factor: 5.958

4.  Pure human butyrylcholinesterase hydrolyzes octanoyl ghrelin to desacyl ghrelin.

Authors:  Lawrence M Schopfer; Oksana Lockridge; Stephen Brimijoin
Journal:  Gen Comp Endocrinol       Date:  2015-06-12       Impact factor: 2.822

5.  Ghrelin is a growth-hormone-releasing acylated peptide from stomach.

Authors:  M Kojima; H Hosoda; Y Date; M Nakazato; H Matsuo; K Kangawa
Journal:  Nature       Date:  1999-12-09       Impact factor: 49.962

6.  The C-terminal T peptide of acetylcholinesterase enhances degradation of unassembled active subunits through the ERAD pathway.

Authors:  Stéphanie Belbeoc'h; Jean Massoulié; Suzanne Bon
Journal:  EMBO J       Date:  2003-07-15       Impact factor: 11.598

7.  Orexigenic action of peripheral ghrelin is mediated by neuropeptide Y and agouti-related protein.

Authors:  H Y Chen; M E Trumbauer; A S Chen; D T Weingarth; J R Adams; E G Frazier; Z Shen; D J Marsh; S D Feighner; X-M Guan; Z Ye; R P Nargund; R G Smith; L H T Van der Ploeg; A D Howard; D J MacNeil; S Qian
Journal:  Endocrinology       Date:  2004-02-12       Impact factor: 4.736

8.  The orexigenic hormone ghrelin defends against depressive symptoms of chronic stress.

Authors:  Michael Lutter; Ichiro Sakata; Sherri Osborne-Lawrence; Sherry A Rovinsky; Jason G Anderson; Saendy Jung; Shari Birnbaum; Masashi Yanagisawa; Joel K Elmquist; Eric J Nestler; Jeffrey M Zigman
Journal:  Nat Neurosci       Date:  2008-06-15       Impact factor: 24.884

9.  Ghrelin and eating disturbances in psychiatric disorders.

Authors:  A Schanze; U Reulbach; M Scheuchenzuber; M Groschl; J Kornhuber; T Kraus
Journal:  Neuropsychobiology       Date:  2008-06-13       Impact factor: 2.328

Review 10.  Ghrelin signalling on food reward: a salient link between the gut and the mesolimbic system.

Authors:  M Perello; S L Dickson
Journal:  J Neuroendocrinol       Date:  2015-06       Impact factor: 3.627
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  5 in total

1.  Potential anti-obesity effects of a long-acting cocaine hydrolase.

Authors:  Xirong Zheng; Jing Deng; Ting Zhang; Jianzhuang Yao; Fang Zheng; Chang-Guo Zhan
Journal:  Chem Biol Interact       Date:  2016-05-06       Impact factor: 5.192

Review 2.  Physiological roles for butyrylcholinesterase: A BChE-ghrelin axis.

Authors:  Stephen Brimijoin; Vicky Ping Chen; Yuan-Ping Pang; Liyi Geng; Yang Gao
Journal:  Chem Biol Interact       Date:  2016-02-23       Impact factor: 5.192

3.  Butyrylcholinesterase Deficiency Promotes Adipose Tissue Growth and Hepatic Lipid Accumulation in Male Mice on High-Fat Diet.

Authors:  Vicky Ping Chen; Yang Gao; Liyi Geng; Michael B Stout; Michael D Jensen; Stephen Brimijoin
Journal:  Endocrinology       Date:  2016-06-14       Impact factor: 4.736

4.  Butyrylcholinesterase gene transfer in obese mice prevents postdieting body weight rebound by suppressing ghrelin signaling.

Authors:  Vicky Ping Chen; Yang Gao; Liyi Geng; Stephen Brimijoin
Journal:  Proc Natl Acad Sci U S A       Date:  2017-09-25       Impact factor: 11.205

5.  Butyrylcholinesterase regulates central ghrelin signaling and has an impact on food intake and glucose homeostasis.

Authors:  V P Chen; Y Gao; L Geng; S Brimijoin
Journal:  Int J Obes (Lond)       Date:  2017-05-22       Impact factor: 5.095
  5 in total

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