Jae-Young Park1, Ji-Youn Sung2, Juhie Lee2, Yong-Koo Park2, Youn Wha Kim2, Gou Young Kim3, Kyu Yeoun Won3, Sung-Jig Lim4. 1. Department of Pathology, Hallym University Chuncheon Sacred Heart Hospital, Chuncheon, Republic of Korea. 2. Department of Pathology, Kyung Hee University Hospital, Seoul, Republic of Korea. 3. Department of Pathology, Kyung Hee University Hospital at Gangdong, Seoul, Republic of Korea. 4. Department of Pathology, Kyung Hee University Hospital at Gangdong, Seoul, Republic of Korea. Electronic address: sungjig@khu.ac.kr.
Abstract
PURPOSE: Tumor-associated macrophages (TAMs) play a significant role in tumor progression and angiogenesis. However, the prognostic value of TAMs in different histologic locations of gastric cancer (GC) is still unknown. We evaluated the distribution of TAMs in different histologic locations to investigate its importance in predicting prognosis and the relationship with angiogenesis and CXCL12 expression in GC. METHODS AND MATERIALS: The distribution of TAMs and microvessel density (MVD) in 113 GC samples were evaluated by immunohistochemical staining of CD163 and CD105, respectively. The extent of TAM distribution in the tumor was categorized into three groups: infiltrated TAMs in the tumor nest (TN), tumor stroma (TS) and invasive tumor margin (TM). The expression of CXCL12 in GC were evaluated by immunohistochemical staining of tissues from 88 GC samples. RESULTS: The increased CD163+ TAMs in TS and TM were closely correlated with tumor size, depth of invasion, TNM stage, lymph node metastasis, and lymphovascular invasion. TAMs in TN was not related with any clinicopathologic characteristics except histologic differentiation. The high infiltration of CD163+ TAMs in TS and TM were significantly correlated with poor overall survival. Regardless of location, CD163+ TAMs were significantly correlated with increased MVD. CXCL12 expression was significantly associated with increased CD163+ TAMs in TS and TM. CONCLUSIONS: TAMs in different histologic locations in GC were related to distinct aspects of tumor progression. CD163+ TAMs in TS and TM are associated with tumor progression and CXCL12 expression in GC. TAMs may be involved in tumor progression through the angiogenesis.
PURPOSE:Tumor-associated macrophages (TAMs) play a significant role in tumor progression and angiogenesis. However, the prognostic value of TAMs in different histologic locations of gastric cancer (GC) is still unknown. We evaluated the distribution of TAMs in different histologic locations to investigate its importance in predicting prognosis and the relationship with angiogenesis and CXCL12 expression in GC. METHODS AND MATERIALS: The distribution of TAMs and microvessel density (MVD) in 113 GC samples were evaluated by immunohistochemical staining of CD163 and CD105, respectively. The extent of TAM distribution in the tumor was categorized into three groups: infiltrated TAMs in the tumor nest (TN), tumor stroma (TS) and invasive tumor margin (TM). The expression of CXCL12 in GC were evaluated by immunohistochemical staining of tissues from 88 GC samples. RESULTS: The increased CD163+ TAMs in TS and TM were closely correlated with tumor size, depth of invasion, TNM stage, lymph node metastasis, and lymphovascular invasion. TAMs in TN was not related with any clinicopathologic characteristics except histologic differentiation. The high infiltration of CD163+ TAMs in TS and TM were significantly correlated with poor overall survival. Regardless of location, CD163+ TAMs were significantly correlated with increased MVD. CXCL12 expression was significantly associated with increased CD163+ TAMs in TS and TM. CONCLUSIONS:TAMs in different histologic locations in GC were related to distinct aspects of tumor progression. CD163+ TAMs in TS and TM are associated with tumor progression and CXCL12 expression in GC. TAMs may be involved in tumor progression through the angiogenesis.
Authors: Francesca Maria Consonni; Augusto Bleve; Maria Grazia Totaro; Mariangela Storto; Paolo Kunderfranco; Alberto Termanini; Fabio Pasqualini; Chiara Alì; Chiara Pandolfo; Francesco Sgambelluri; Giulia Grazia; Mario Santinami; Andrea Maurichi; Massimo Milione; Marco Erreni; Andrea Doni; Marco Fabbri; Laura Gribaldo; Eliana Rulli; Miguel Parreira Soares; Valter Torri; Roberta Mortarini; Andrea Anichini; Antonio Sica Journal: Nat Immunol Date: 2021-04-26 Impact factor: 25.606