Agnese Veronesi1, Cristina Rota2, Tommaso Trenti2, Elisabetta Cariani2. 1. Laboratory of Toxicology, Department of Laboratory Medicine, Ospedale S. Agostino-Estense, Modena, Italy. ag.veronesi@ausl.mo.it. 2. Laboratory of Toxicology, Department of Laboratory Medicine, Ospedale S. Agostino-Estense, Modena, Italy.
Abstract
BACKGROUND: Carbohydrate-deficient transferrin (CDT) is used to assess chronic alcohol consumption in administrative and forensic context. The aim of the present study was the optimization of the diagnostic strategy for CDT determination in a clinical laboratory setting. METHODS: Two capillary zone electrophoresis (CZE) assays, the CEofix CDT (Analis, Suarlée, Belgium) run on single capillary MDQ instrument and the muticapillary (Sebia, Lisses, France), were compared as screening methods and a commercial high-performance liquid chromatography (HPLC) assay (Recipe, Munich, Germany) was used for confirmation. RESULTS: In total, 367 serum samples were analyzed by both CZE assays with concordant classification in 92% of cases. All discordant samples were classified as negative by HPLC, as did 2/3 of those that could not be classified by either CZE assay. Classification of samples with CDT values close to cut-off by CZE was confirmed by HPLC in 95-100% of negative samples but only in 28.6-33.3% of positive samples. CONCLUSIONS: Both CZE assays proved suitable for CDT screening. HPLC was useful for discriminating CDT value in most of samples that could not be interpreted by CZE due to analytical interferences. Considering the implication of CDT testing, HPLC assay may also be helpful for the confirmation of positive results close to the cut-off value of CZE assays.
BACKGROUND:Carbohydrate-deficient transferrin (CDT) is used to assess chronic alcohol consumption in administrative and forensic context. The aim of the present study was the optimization of the diagnostic strategy for CDT determination in a clinical laboratory setting. METHODS: Two capillary zone electrophoresis (CZE) assays, the CEofix CDT (Analis, Suarlée, Belgium) run on single capillary MDQ instrument and the muticapillary (Sebia, Lisses, France), were compared as screening methods and a commercial high-performance liquid chromatography (HPLC) assay (Recipe, Munich, Germany) was used for confirmation. RESULTS: In total, 367 serum samples were analyzed by both CZE assays with concordant classification in 92% of cases. All discordant samples were classified as negative by HPLC, as did 2/3 of those that could not be classified by either CZE assay. Classification of samples with CDT values close to cut-off by CZE was confirmed by HPLC in 95-100% of negative samples but only in 28.6-33.3% of positive samples. CONCLUSIONS: Both CZE assays proved suitable for CDT screening. HPLC was useful for discriminating CDT value in most of samples that could not be interpreted by CZE due to analytical interferences. Considering the implication of CDT testing, HPLC assay may also be helpful for the confirmation of positive results close to the cut-off value of CZE assays.
Authors: Cas Weykamp; Jos Wielders; Anders Helander; Raymond F Anton; Vincenza Bianchi; Jan-Olof Jeppsson; Carla Siebelder; John B Whitfield; François Schellenberg Journal: Clin Chem Date: 2014-04-01 Impact factor: 8.327
Authors: Cas Weykamp; Jos P M Wielders; Anders Helander; Raymond F Anton; Vincenza Bianchi; Jan-Olof Jeppsson; Carla Siebelder; John B Whitfield; François Schellenberg Journal: Clin Chem Lab Med Date: 2013-05 Impact factor: 3.694
Authors: Manuela G Neuman; Helmut K Seitz; Rolf Teschke; Stephen Malnick; Kamisha L Johnson-Davis; Lawrence B Cohen; Anit German; Nicolas Hohmann; Bernhardo Moreira; George Moussa; Mihai Opris Journal: Curr Issues Mol Biol Date: 2022-03-16 Impact factor: 2.976