Regina M Krohn1, Maryse Lemaire2, Luis F Negro Silva3, Catherine Lemarié4, Alicia Bolt2, Koren K Mann2, Judit E Smits5. 1. Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, T2N 4Z6, Canada. Electronic address: regina.mkrohn@gmail.com. 2. Department of Oncology, Lady Davis Institute for Medical Research, McGill University, Montréal, H3T 1E2, Canada. 3. Experimental Medicine, Lady Davis Institute for Medical Research, McGill University, Montréal, H3T 1E2, Canada. 4. Department of Medicine, Lady Davis Institute for Medical Research, McGill University, Montréal, H3T 1E2, Canada. 5. Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, T2N 4Z6, Canada.
Abstract
BACKGROUND: Cardiovascular disease (CVD) is a major cause of death worldwide, and arsenic (As) intake, mainly through drinking water, is a well-known risk factor for CVD as well as other health problems. Selenium (Se) is a known antagonist to As toxicity. OBJECTIVE: We tested the potential of high-Se lentils from the Canadian prairies as a therapeutic food to alter the outcome of As-enhanced atherosclerosis. MATERIALS AND METHODS: Male ApoE(-/-) mice exposed to a moderate level of As (200ppb) in their drinking water, and control mice on tap water received one of three lentil diets: Se-deficient (0.009mg/kg), Se-adequate (0.16mg/kg) or Se-high (0.3mg/kg). After 13weeks, lesion formation in the aortic arch and sinus were assessed. Intralesional cellular composition, serum lipid levels and hepatic oxidative stress were assessed as well. RESULTS: Arsenic-exacerbated plaque formation was reduced in the sinus and completely abolished in the aortic arch of mice on the Se-fortified lentil diet, whereas lesions were increased in As-exposed mice on both the Se-deficient and Se-adequate diets. Notably, Se deficiency contributed to proatherogenic composition of serum lipids in As-exposed mice as indicated by high-density lipoprotein:low-density lipoprotein. At least adequate Se status was crucial for counteracting As-induced oxidative stress. CONCLUSION: This study is the first to show the potential of high-Se lentils to protect against As-triggered atherosclerosis, and this invites further investigations in human populations at risk from As contamination of their drinking water. Crown
BACKGROUND:Cardiovascular disease (CVD) is a major cause of death worldwide, and arsenic (As) intake, mainly through drinking water, is a well-known risk factor for CVD as well as other health problems. Selenium (Se) is a known antagonist to Astoxicity. OBJECTIVE: We tested the potential of high-Selentils from the Canadian prairies as a therapeutic food to alter the outcome of As-enhanced atherosclerosis. MATERIALS AND METHODS: Male ApoE(-/-) mice exposed to a moderate level of As (200ppb) in their drinking water, and control mice on tapwater received one of three lentil diets: Se-deficient (0.009mg/kg), Se-adequate (0.16mg/kg) or Se-high (0.3mg/kg). After 13weeks, lesion formation in the aortic arch and sinus were assessed. Intralesional cellular composition, serum lipid levels and hepatic oxidative stress were assessed as well. RESULTS:Arsenic-exacerbated plaque formation was reduced in the sinus and completely abolished in the aortic arch of mice on the Se-fortified lentil diet, whereas lesions were increased in As-exposed mice on both the Se-deficient and Se-adequate diets. Notably, Se deficiency contributed to proatherogenic composition of serum lipids in As-exposed miceas indicated by high-density lipoprotein:low-density lipoprotein. At least adequate Se status was crucial for counteracting As-induced oxidative stress. CONCLUSION: This study is the first to show the potential of high-Selentils to protect against As-triggered atherosclerosis, and this invites further investigations in human populations at risk from As contamination of their drinking water. Crown
Authors: Maryse Lemaire; Luis Fernando Negro Silva; Catherine A Lemarié; Alicia M Bolt; Manuel Flores Molina; Regina M Krohn; Judit E Smits; Stéphanie Lehoux; Koren K Mann Journal: PLoS One Date: 2015-09-02 Impact factor: 3.240
Authors: Luis Fernando Negro Silva; Maryse Lemaire; Catherine A Lemarié; Dany Plourde; Alicia M Bolt; Christopher Chiavatti; D Scott Bohle; Vesna Slavkovich; Joseph H Graziano; Stéphanie Lehoux; Koren K Mann Journal: Environ Health Perspect Date: 2017-07-05 Impact factor: 9.031