Literature DB >> 2649493

Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids.

H Flinn1, M Burke, C J Stirling, D J Sherratt.   

Abstract

Escherichia coli mutants completely defective in ColE1 cer-mediated site-specific recombination have been mapped to two genes, xerA and xerB. In this study, xerA xerB double mutants were constructed by gene replacement with a lambda dv plasmid and were shown to be both viable and defective in ColE1 site-specific recombination.

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Year:  1989        PMID： 2649493      PMCID： PMC209887          DOI： 10.1128/jb.171.4.2241-2243.1989

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  16 in total

Review 1.  Site-specific recombinases: changing partners and doing the twist.

Authors:  P Sadowski
Journal:  J Bacteriol       Date:  1986-02       Impact factor: 3.490

2.  Multimer resolution systems of ColE1 and ColK: localisation of the crossover site.

Authors:  D Summers; S Yaish; J Archer; D Sherratt
Journal:  Mol Gen Genet       Date:  1985

3.  Trimethoprim resistance conferred by W plasmids in Enterobacteriaceae.

Authors:  N Datta; R W Hedges
Journal:  J Gen Microbiol       Date:  1972-09

4.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors:  J Vieira; J Messing
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

5.  Multimerization of high copy number plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability.

Authors:  D K Summers; D J Sherratt
Journal:  Cell       Date:  1984-04       Impact factor: 41.582

6.  Maintenance of multicopy plasmid Clo DF13 in E. coli cells: evidence for site-specific recombination at parB.

Authors:  M J Hakkaart; P J van den Elzen; E Veltkamp; H J Nijkamp
Journal:  Cell       Date:  1984-01       Impact factor: 41.582

7.  Rosanilins: indicator dyes for chloramphenicol-resistant enterobacteria containing chloramphenicol acetyltransferase.

Authors:  G N Proctor; R H Rownd
Journal:  J Bacteriol       Date:  1982-06       Impact factor: 3.490

8.  Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.

Authors:  E Amann; J Brosius; M Ptashne
Journal:  Gene       Date:  1983-11       Impact factor: 3.688

9.  An Escherichia coli mutant unable to support site-specific recombination of bacteriophage lambda.

Authors:  A Kikuchi; E Flamm; R A Weisberg
Journal:  J Mol Biol       Date:  1985-05-25       Impact factor: 5.469

10.  Replacement and amplification of bacterial genes with sequences altered in vitro.

Authors:  N I Gutterson; D E Koshland
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205
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  3 in total

1.  Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases.

Authors:  S D Colloms; P Sykora; G Szatmari; D J Sherratt
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

2.  Site-specific recombination between ColE1 cer and NTP16 nmr sites in vivo.

Authors:  N Zakova; G B Szatmari
Journal:  Mol Gen Genet       Date:  1995-05-20

3.  Low-level expression of the Type II restriction-modification system confers potent bacteriophage resistance in Escherichia coli.

Authors:  Karolina Wilkowska; Iwona Mruk; Beata Furmanek-Blaszk; Marian Sektas
Journal:  DNA Res       Date:  2020-02-01       Impact factor: 4.458
  3 in total

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