Are invasive plants more competitive than native conspecifics? Patterns vary with competitors.

Invasive plants are sometimes considered to be more competitive than their native conspecifics, according to the prediction that the invader reallocates resources from defense to growth due to liberation of natural enemies ['Evolution of Increased Competitive Ability' (EICA) hypothesis]. However, the differences in competitive ability may depend on the identity of competitors. In order to test the effects of competitors, Ageratina adenophora plants from both native and invasive ranges competed directly, and competed with native residents from both invasive (China) and native (Mexico) ranges respectively. Invasive A. adenophora plants were more competitive than their conspecifics from native populations when competing with natives from China (interspecific competition), but not when competing with natives from Mexico. Invasive A. adenophora plants also showed higher competitive ability when grown in high-density monoculture communities of plants from the same population (intrapopulation competition). In contrast, invasive A. adenophora plants showed lower competitive ability when competing with plants from native populations (intraspecific competition). Our results indicated that in the invasive range A. adenophora has evolved to effectively cope with co-occurring natives and high density environments, contributing to invasion success. Here, we showed the significant effects of competitors, which should be considered carefully when testing the EICA hypothesis.

It is frequently reported that invasive plants have a great impact on species composition, plant community structure, and ecosystem function, and that the invaders from invasive ranges are more competitive than their conspecifics from native ranges12. It has been proposed that once a plant is introduced into a new range, novel selection pressures from biotic and abiotic factors may induce evolutionary changes34. This may lead to ecologically important differentiation between plants from native and invasive populations. The Evolution of Increased Competitive Ability (EICA) hypothesis predicts that exotic plants may escape from the control of natural enemies in introduced ranges and gradually evolve to lose costly defense traits, reallocating resources and energy from defense to growth5. Siemann and Rogers678 found that Sapium sebiferum plants from invasive populations have higher competitive ability and lower leaf defensive ability than plants from native populations. Huang et al.9 also found that Triadica sebifera plants from invasive populations have higher biomass and lower defense to herbivores compared to plants from native populations.

The results for some invasive plants have also been inconsistent. Vilà et al.10 found that plants from invasive populations of Hypericum perforatum are not better competitors than plants from native populations. Senecio pterophorus plants grow similarly in the native and invasive ranges11, while the competitive ability of Alliaria petiolata plants from invasive populations is lower than that of plants from native populations12. Competitive conditions have significant impacts on the relative performance of plants from native and invasive ranges13. Leger and Rice14 found that introduced Eschscholzia californica from Chile is larger and more fecund than native Californian conspecifics only in the absence of competition. In contrast, Bossdorf et al.12 found that performance is similar for Alliaria petiolata plants from native and invasive populations in the absence of competition, whereas native plants outperform their invasive conspecifics when competing against each other. Increased or equal competitive ability has also been found for plants from invasive populations of other invaders (Table 1). The recent studies revealed that evolution indeed happened in invasive plants but little support for EICA hypothesis1516.

Table 1

Differences in competitive ability between plants originated from invasive and native ranges of eight species reported in references.

Species	Competitive ability	Competitors	References
Eschscholzia californica	No	IN	Leger and Rice (2003)14
Hypericum perforatum	No	IN	Vilà et al. (2003)10
Alliaria petiolata	Decrease	D	Bossdorf et al. (2004)12
Silene latifolia	No	INV	Blair and Wolfe (2004)34
Centaurea maculosa	Increase	IV	Ridenour et al. (2008)35
Dactylis glomerata	Decrease	D	Liesfo et al. (2012)36
Solidago canadensis	Increase	IV	Yuan et al. (2013)31
Chromolaena odorata	Increase	D	Zheng et al. (2015)18

“Increase” indicates higher competitive ability for plants from invasive populations compared with plants from native populaitons; “Decrease” indicates lower competitive ability for plants from invasive populations; “No” indicates similar competitive ability between plants from invasive and native populations. “IN” indicates that the competitors are resident native species from native range; “IV” indicates that the competitors are resident native species from invasive range; “INV” indicates that the competitors are resident native species from both ranges; “D” indicates that plants from invasive populations were competed with plants from native populations.

Table 2

Differences in aboveground biomass when grown in monoculture between native and invasive ranges, and differences in change in aboveground biomass between native and invasive ranges when grown in intraspecific competition, interspecific competition and high density plantation according to one-way nested ANOVAs analysis.

Experiment	Variable	df	F-value	P
Monoculture	Aboveground biomass	1	6.555	0.034
Intraspecific competition	Change in Aboveground biomass	1	10.183	0.013
Interspecific competitionv
with native species from Mexico	Change in Aboveground biomass	1	0.413	0.528
with native species from China	Change in Aboveground biomass	1	5.629	0.029
High density plantation	Change in Aboveground biomass	1	35.4	<0.001

The identity of competitors may influence the results of competitive experiments17. Native plants from the native range of an invasive plant may have adapted to the presence of the invader as they have a long co-evolutionary history; therefore, they may be less vulnerable to competition than natives from the invasive range of the invader. Under such circumstance, using native species from the native and invasive ranges of the invader as competitors may result in different conclusions regarding the intraspecific difference in the competitive ability of the invader. Similarly, the results of intraspecific competition may also differ with those of interspecific competition. Successful invasive plants often form dense monocultures in invasive ranges, whereas native conspecifics remain sparsely distributed in native ranges18. Thus, comparison with grown in monoculture, high density plantation might have less effects on invasive plants from the invasive ranges than native conspecifics.

In this study, we explored the effects of different competitors (natives from both ranges of the invader and the invader itself) on intraspecific differences in the competitive ability of the invasive A. adenophora using common garden experiments. A. adenophora is a perennial forb, native to Central America and Mexico, but a noxious invasive species in southern and southeastern Asia, eastern Australia, New Zealand, and southwestern Africa19. We studied performance differences between A. adenophora plants from native and invasive ranges in the absence of competitors (monoculture experiment), in the presence of intraspecific competitors (intraspecific competition experiment) and interspecific competitors from both ranges of the invader (interspecific competition experiment), and in an artificial monoculture community with high density (high density experiment). We also addressed the following questions: (1) Does A. adenophora gain or lose competitive ability against native species in its invasive range, (2) Does invasive A. adenophora also gain or lose intraspecific competitive ability, and (3) Does invasive A. adenophora also gain or lose competitive ability against native species in its original range?

Results

The identity of competitors had a significant effect on the competitive ability of native and invasive A. adenophora plants. When grown in monoculture, total biomass was not significantly different between A. adenophora plants from native and invasive ranges (Appendix 1a), although the plants from invasive populations produced more aboveground biomass (Fig. 1a; Table 2). The A. adenophora plants from invasive populations had lower root biomass fraction than those from the native range (Appendix 1b). When invasive A. adenophora plants competed with their native conspecifics (intraspecific competition), the decrease in aboveground biomass was significantly higher for the plants from invasive populations (Fig. 1b; Table 2).

Figure 1

Aboveground biomass of Ageratina adenophora plants from the native (closed bars) and invasive (open bars) populations grown in monoculture (a), and changes in aboveground biomass caused by intraspecific competition (b). Narrow bars indicate means and SE for each population; two thicker bars in the center depict means and SE for each range. * indicates significant differences between ranges (P < 0.05) in aboveground biomass (one-way nested ANOVAs) and percentage change in aboveground biomass (t-test).

When grown in monoculture, the aboveground biomass of the two native species from the invasive range of the invader (China) was significantly lower than that of A. adenophora plants from both native and invasive ranges (Appendix 2a). However, the differences in aboveground biomass were not significantly different between the two native species from the native range of the invader (Mexico) and A. adenophora from both ranges (Appendix 3a). Competition from the natives of both ranges (interspecific competition) decreased the aboveground biomass of A. adenophora from both ranges, and the decrease was significantly higher when the natives from Mexico were used as competitors (Fig. 2; Table 2). However, the differences in competitive ability were not significant between A. adenophora plants from native and invasive populations when competing with the two natives from Mexico (Fig. 2; Table 2; Appendix 3a). When they competed with the natives from China, A. adenophora plants from invasive populations had significantly higher competitive ability than those from native populations (Fig. 2; Appendix. 2b). Compared to A. adenophora from both ranges, the interspecific competitive ability was lower for the natives from China, but higher for the natives from Mexico (Appendix 2b, 3b).

Figure 2

Changes in aboveground biomass of Ageratina adenophora plants from the native (closed bars) and invasive (open bars) populations caused by competition of two resident native species from Mexico (native range) and China (invasive range), respectively. * indicates significant differences between ranges (P < 0.05) (one-way nested ANOVAs).

When grown at high individual density (intrapopulation competition), the decrease in aboveground biomass was higher for A. adenophora plants from native populations than those from invasive populations (Fig. 3; Table 2).

Figure 3

Changes in aboveground biomass of Ageratina adenophora plants from the native (closed bars) and invasive (open bars) populations when grown in artificial communities with high density. Narrow bars indicate means and SE for each population; two thicker bars in the center depict means and SE for each range. * indicates significant differences between ranges (P < 0.05) (one-way nested ANOVAs).

Discussion

Our results indicated that the differences in competitive ability between A. adenophora plants from native and invasive populations were inconsistent, depending on the competitors used in our common garden experiment. Thus, it is important to take into account the effects of competitors when testing the EICA (Evolution Increased Competitive Ability) hypothesis, choose suitable competitors according to the specific purpose, and explain experimental results carefully. In addition, abiotic environments also influence experimental results13.

Higher aboveground biomass did not lead to higher intraspecific competitive ability for A. adenophora plants from the invasive range, results that were inconsistent with the prediction of the EICA hypothesis51718. The lower intraspecific competitive ability of A. adenophora plants from the invasive range may be associated with their lower root mass fraction (Appendix 1b). Several studies have also found that the competitive advantages of invasive species are associated with shifts in biomass allocation rather than increased individual size20212223. The decreased biomass allocation to roots in invasive populations of A. adenophora may be associated with improved soil environments (moisture, nutrients, or microbes) in the invasive range. Annual precipitation was significantly higher in the invasive range than in the native range (Appendix 4). Plants generally decrease biomass allocation to roots in benign belowground conditions2425. Reallocation of biomass from roots to aboveground parts allows invasive A. adenophora to be more effective in competing for light. Natural selection may favor genotypes with increased light capture ability in invasive ranges with increased precipitation, contributing to successful competition with native species. However, the lower root mass fraction may be a disadvantage of A. adenophora in native ranges with lower precipitation. Thus, in our study site located in Tlayacapan, Mexico, A. adenophora plants from the native range with higher root mass fraction showed higher competitive ability than those from the invasive range with lower root mass fraction.

The higher interspecific competitive ability of A. adenophora plants from the invasive range when they competed with the natives from the invasive range (China) of the invader may be associated with their greater aboveground biomass and stronger allelopathic effects compared to those from the native range. The natives from China grew more slowly than A. adenophora plants from both ranges (Appendix 2). When competing with the slowly growing natives from China, the higher biomass allocation to shoot might provide a competitive advantage to the invasive A. adenophora compared to its native conspecifics20. It has been reported that the invader may use dense canopy to outshade competitors in the invasive range26. Previous studies have demonstrated that A. adenophora has strong allelopathic effects on neighboring plants2728. Consistently with the novel weapons hypothesis1718, native species from the invasive range of A. adenophora were more vulnerable to the allelochemicals of the invader than the natives from the native range of the invader29. In this case, natural selection may favor the genotypes with increased allelopathic effects in the invasive range of A. adenophora. Concentrations of some allelochemicals were indeed higher in A. adenophora plants from the invasive range than in those from the native range29. The higher allelopathic effect of A. adenophora plants from the invasive range may contribute to higher competitive ability when competing with natives from China, which are vulnerable to the allelochemicals of the invader. Increased allelochemical-driven competitive advantage was also found in other invasive plants18.

However, the greater aboveground biomass and stronger allelopathic effects of A. adenophora plants from the invasive range did not lead to higher interspecific competitive ability when they competed with the natives (Cosmos sulphureus and Aldama dentata) from the native range (Mexico) of the invader.It might due to C. sulphureus and A. dentata have long co-evolutionar history with A. adenophora, they might not be sensitive to the allelochemicals of A. adenophora. So, stronger allelopathic effects of A. adenophora plants from the invasive range did not contribute to higher competitive ability. In addition, C. sulphureus and A. dentata grew much faster than A. adenophora in the early period of the interspecific competition experiment, although the final aboveground biomass was similar among species. The biomass of the natives from Mexico almost reached its final values in 3 months (personal observation). The faster growth rates of the natives from Mexico not only contributed to their higher competitive ability compared to A. adenophora from both ranges (Appendix 3b), but also eliminated the competitive advantage of A. adenophora plants from the invasive range. The increased aboveground biomass did not increase the competitive ability of A. adenophora plants from the invasive range as they grew under the canopy of the natives from Mexico, and could not effectively shade the competitors.In addition, the stronger allelopathic effects of A. adenophora plants from the invasive range might not increase their competitive ability when competing with natives from Mexico, which might not vulnerable to allelochemicals of the invader as they share long co-evolutionary history.

In the native range, A. adenophora plants are often sparsely distributed, whereas they generally form dense monocultures in the invasive range (personal observation). A. adenophora may have evolved certain adaptive strategies (morphological or physiological) in the invasive range to cope with high-density environments. Therefore, the decrease in aboveground biomass was less in A. adenophora plants from the invasive range than in those from the native range when grown in high-density (Fig. 3).

Many factors can cause evolutionary changes in invasive plants, for example novel assemblages of enemies and plants and new abiotic environments in introduced ranges5123031. Novel biotic and abiotic environments may lead to new competitive strategies for invasive plants, which may be quite different from those that they have in their native ranges. The differences in competitive ability between plants from native and invasive ranges may be different when different competitors are used (Figs 1, 2, 3) or under different abiotic conditions13.

In conclusion, A. adenophora plants from the invasive range showed higher interspecific competitive ability than those from the native range when competing with native species from the invasive range of the invader, but not when competing with native species from the native range of the invader. Plants from the invasive range of the invader also showed higher intrapopulation competitive ability when grown in high density. The increased ability to deal with co-occurring natives and strongly competitive environments may contribute to the success of the invader in the invasive range. In contrast, A. adenophora plants from the invasive range showed lower intraspecific competitive ability. Our results indicated that the differences in competitive ability between plants from native and invasive populations are competitor-dependent, which should be considered when testing the EICA hypothesis.

Methods

Study sites and materials

This study was conducted within the native range of A. adenophora in Tlayacapan, Morelos, Mexico (18°57′N, 98°58′W; 1634 m above sea level). The mean annual temperature of this area is 19.3 °C, the mean temperature of the hottest month (June) is 22.9 °C, and the mean temperature of the coolest month (January) is 16.9 °C. The mean annual precipitation is 988 mm with a dry period from November to April32.

In 2006, we collected seeds of A. adenophora from five populations in the native range (Mexico) and five populations in the invasive range (three in China and two in India) (Appendix 5). Seeds were collected from more than 10 individuals that were at least 20 m apart from one another. In order to exclude maternal effect, we used seeds of the next generation. Seeds were germinated in a seedbed in December 2006. In February 2007, when the seedlings were approximately 10 cm tall, 200 similarly sized seedlings (20 per population) were planted in a common garden located in Menglun, Mengla County, Yunnan Province, southwest China (21°56′N, 101°150′E; 570 m above sea level). The reproduction system of A. adenophora is apomixis33, which avoids hybridization among different populations. In May 2008, A. adenophora seeds of each population were collected.

In 2009, we collected seeds from the native Cosmos sulphureus and Aldama dentata in the native range of A. adenophora (Mexico) and from the native Eupatorium japonicum and E. stoechadosmum in the invasive range of A. adenophora (China). All these species were sympatric and ecologically similar to A. adenophora. For each of these species, seeds were also collected from more than 10 individuals.

Common garden experiments

In April 2010, seeds of A. adenophora from each population, and the four natives from Mexico and China were sown separately into a seed bed in a greenhouse in Tlayacapan. In June 2010, similar-sized vigorous seedlings (approximately 10 cm tall) were transplanted into a common garden, in which we established 69 rectangular plots (6.5 m × 60 cm) and 18 square plots (1 m × 1 m). The seedlings of A. adenophora were grown under four conditions: monoculture, intraspecific competition, interspecific competition, and high density (Appendix 6).

For monoculture, we used 10 rectangular plots and in each of those we grew one seedling of A. adenophora from each population (10 replicates). Four rectangular plots were used for monoculture of seedlings of four native species. Due to the limited number of seedlings, for intraspecific competition, we grew seedlings of A. adenophora from each native population with one seedling from the three randomly selected invasive populations (6 cm apart between competitors). There were three competition combinations for each population and 15 combinations in total. Each rectangular plot contained 10 different competing pairs, and there were 10 replicates for each competing pair (150 competing pairs in total grown in 15 rectangular plots). For interspecific competition, we grew one seedling of A. adenophora from each population with one seedling from each of the four native species. There were also 10 replicates for each competing pair (400 competing pairs in total grown in 40 rectangular plots). Individual seedlings or competing pairs were planted 60 cm apart from any other seedling or seedling pair. The 65 rectangular plots were randomly distributed in the garden (Appendix 6). Due to the limited number of seedlings for several populations of A. adenophora, seedlings of A. adenophora from only six populations (three invasive and three native) were used in the artificial monoculture community with high density. Forty-nine seedlings from each of the six populations were transplanted into a square plot (1 m × 1 m), and there were three replicates for each population (18 square plots). These 18 square plots, which were randomly distributed in the garden (Appendix 6), were used to mimic communities that are dominated by A. adenophora at high density.

In February 2011, the aboveground parts of all plants were harvested, oven-dried at 60 °C for 48 h, and weighed. In order to avoid border effect in the high-density growth experiment, 10 A. adenophora seedlings were randomly harvested in the center part of each plot. In order to test whether biomass reallocation occurred after introduction, roots of A. adenophora plants from both ranges grown in monoculture were collected, washed, oven-dried at 60 °C for 48 h, and weighed. Root mass fraction was calculated as the ratio of root mass to total mass. Roots were not collected for plants grown in under intraspecific and interspecific competition and high density because the roots of two or more individuals often twined together, increasing the difficulty to separate the roots of different individual.

Response to competition was measured as the percentage change in aboveground biomass, i.e., [(Biomasscomp − Biomassmono)/Biomassmono] × 100%, where Biomassmono is the mean aboveground biomass of A. adenophora plants from each population or each of the four native species grown in monoculture, and Biomasscomp is the mean aboveground biomass of A. adenophora plants from each population or each of the four native species when grown with competitors.

Statistical analysis

The differences in total biomass, aboveground biomass, and root mass fraction between A. adenophora plants from native and invasive ranges grown in monoculture were tested using one-way nested ANOVA (Fig. 1a; Appendix 1). Range was used as a fixed factor, and population nested within range was used as a random factor. The differences in the percentage change of aboveground biomass caused by intraspecific competition between A. adenophora plants from native and invasive ranges were compared using t-tests (Fig. 1b). The differences in the percentage changes of aboveground biomass between A. adenophora plants from native and invasive ranges grown in high density were tested using one-way nested ANOVA (Fig. 3). Range was used as a fixed factor, and population nested within range was used as a random factor. The difference in aboveground biomass and the percentage change of biomass between native and invasive populations of A. adenophora species native to China or Mexico was determined using one-way ANOVA (Fig. 2; Appendix 2, 3). The differences in annual precipitation between ranges were tested using one-way ANOVA (Appendix 6).

Additional Information

How to cite this article: Zheng, Y. et al. Are invasive plants more competitive than native conspecifics? Patterns vary with competitors. Sci. Rep. 5, 15622; doi: 10.1038/srep15622 (2015).