A C Ionescu1, E Brambilla2, A Travan3, E Marsich4, I Donati5, P Gobbi6, G Turco7, R Di Lenarda8, M Cadenaro9, S Paoletti10, L Breschi11. 1. Department of Biomedical, Surgical and Dental Sciences, IRCCS Galeazzi Institute, University of Milan, via R. Galeazzi, 4, 20133 Milan, Italy; Department of Medical Sciences, University of Trieste, Piazza dell'Ospitale, 1, 34125 Trieste, Italy. Electronic address: andreiionescu_40@hotmail.com. 2. Department of Biomedical, Surgical and Dental Sciences, IRCCS Galeazzi Institute, University of Milan, via R. Galeazzi, 4, 20133 Milan, Italy. Electronic address: eugenio.brambilla@unimi.it. 3. Department of Life Sciences, University of Trieste, via Giorgieri 5, 34127 Trieste, Italy. Electronic address: atravan@units.it. 4. Department of Medical Sciences, University of Trieste, Piazza dell'Ospitale, 1, 34125 Trieste, Italy. Electronic address: emarsich@units.it. 5. Department of Life Sciences, University of Trieste, via Giorgieri 5, 34127 Trieste, Italy. Electronic address: idonati@units.it. 6. Department of Earth, Life and Environmental Sciences (DiSTeVA), University of Urbino, Via Ca' Le Suore 2/4, 61029 Urbino (PU) - Italy. Electronic address: pietro.gobbi@uniurb.it. 7. Department of Medical Sciences, University of Trieste, Piazza dell'Ospitale, 1, 34125 Trieste, Italy. Electronic address: gturco@units.it. 8. Department of Medical Sciences, University of Trieste, Piazza dell'Ospitale, 1, 34125 Trieste, Italy. Electronic address: rdilenarda@units.it. 9. Department of Medical Sciences, University of Trieste, Piazza dell'Ospitale, 1, 34125 Trieste, Italy. Electronic address: mcadenaro@units.it. 10. Department of Life Sciences, University of Trieste, via Giorgieri 5, 34127 Trieste, Italy. Electronic address: paolese@units.it. 11. Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Via San Vitale, 59, 40125 Bologna, Italy. Electronic address: lorenzo.breschi@unibo.it.
Abstract
OBJECTIVES: The aim of this study was to determine the in vitro microbiological performances of a lactose-modified chitosan (Chitlac) coating inside which silver nanoparticles were embedded (Chitlac-nAg) for BisGMA/TEGDMA methacrylic specimens. METHODS: Different concentrations of nAg inside Chitlac coating were tested (1 mM, 2 mM, 5 mM). Specimen surface was analyzed by means of field-emission scanning electron microscopy (FEISEM) and energy-dispersive X-ray spectroscopy (EDS). A 48 h monospecific Streptococcus mutans biofilm was developed over the specimen surfaces using a modified drip-flow bioreactor; adherent viable biomass was assessed by MTT test and biofilm was imaged by confocal laser-scanning microscopy (CLSM). RESULTS: The presence of finely dispersed nanoparticles inside the Chitlac coating was confirmed by FEISEM and EDS analysis. All nanoparticles were embedded in the Chitlac coating layer. Chitlac-nAg coatings were able to significantly decrease biofilm formation depending on the nAg concentration, reaching a -80% viable biomass decrease when the 5 mM nAg-Chitlac group was confronted to non-coated control specimens. CLSM analysis did not provide evidence of a contact-killing activity, however the antibacterial Chitlac-nAg coating was able to alter biofilm morphology preventing the development of mature biofilm structures. CONCLUSIONS: The microbiological model applied in this study helped in assessing the antibacterial properties of a coating designed for methacrylate surfaces. CLINICAL SIGNIFICANCE: A microbiological model based on a bioreactor-grown biofilm is useful for preliminary in vitro tests of dental materials. In translational terms, an antibacterial nanocomposite coating based on Chitlac-nAg and designed to be applied to methacrylic surfaces may be a promising way to obtain dental materials able to actively prevent secondary caries.
OBJECTIVES: The aim of this study was to determine the in vitro microbiological performances of a lactose-modified chitosan (Chitlac) coating inside which silver nanoparticles were embedded (Chitlac-nAg) for BisGMA/TEGDMA methacrylic specimens. METHODS: Different concentrations of nAg inside Chitlac coating were tested (1 mM, 2 mM, 5 mM). Specimen surface was analyzed by means of field-emission scanning electron microscopy (FEISEM) and energy-dispersive X-ray spectroscopy (EDS). A 48 h monospecific Streptococcus mutans biofilm was developed over the specimen surfaces using a modified drip-flow bioreactor; adherent viable biomass was assessed by MTT test and biofilm was imaged by confocal laser-scanning microscopy (CLSM). RESULTS: The presence of finely dispersed nanoparticles inside the Chitlac coating was confirmed by FEISEM and EDS analysis. All nanoparticles were embedded in the Chitlac coating layer. Chitlac-nAg coatings were able to significantly decrease biofilm formation depending on the nAg concentration, reaching a -80% viable biomass decrease when the 5 mM nAg-Chitlac group was confronted to non-coated control specimens. CLSM analysis did not provide evidence of a contact-killing activity, however the antibacterial Chitlac-nAg coating was able to alter biofilm morphology preventing the development of mature biofilm structures. CONCLUSIONS: The microbiological model applied in this study helped in assessing the antibacterial properties of a coating designed for methacrylate surfaces. CLINICAL SIGNIFICANCE: A microbiological model based on a bioreactor-grown biofilm is useful for preliminary in vitro tests of dental materials. In translational terms, an antibacterial nanocomposite coating based on Chitlac-nAg and designed to be applied to methacrylic surfaces may be a promising way to obtain dental materials able to actively prevent secondary caries.
Authors: Marialucia Gallorini; Viviana di Giacomo; Valentina Di Valerio; Monica Rapino; Domenico Bosco; Andrea Travan; Mara Di Giulio; Roberta Di Pietro; Sergio Paoletti; Amelia Cataldi; Silvia Sancilio Journal: J Mater Sci Mater Med Date: 2016-10-27 Impact factor: 3.896
Authors: Rosa Virginia Dutra de Oliveira; Fernanda Salloume Sampaio Bonafé; Denise Madalena Palomari Spolidorio; Cristiane Yumi Koga-Ito; Aline Leite de Farias; Kelly R Kirker; Garth A James; Fernanda Lourenção Brighenti Journal: Microorganisms Date: 2020-01-31