Julieta E Ochoa-Amaya1, Eduardo K Hamasato2, Carla N Tobaruela2, Nicolle Queiroz-Hazarbassanov2, Janete A Anselmo Franci3, João Palermo-Neto2, Flavia R Greiffo4, Auriléia Aparecida de Britto4, Rodolfo Paula Vieira4, Ana P Ligeiro de Oliveira4, Cristina de O Massoco2, Luciano F Felicio5. 1. Departamento de Patologia da Faculdade de Medicina Veterináriae Zootecnia da Universidade de São Paulo, São Paulo, SP, Brazil; Facultad de Ciencias Agropecuarias y Recursos Naturales, Programa de Medicina Veterinaria y Zootecnia, Universidad de los Llanos, Villavicencio, Colombia. 2. Departamento de Patologia da Faculdade de Medicina Veterináriae Zootecnia da Universidade de São Paulo, São Paulo, SP, Brazil. 3. Laboratório de Neuroendocrinologia da Reprodução, Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, São Paulo, SP, Brazil. 4. Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde, Universidade Nove de Julho, São Paulo, Brazil. 5. Departamento de Patologia da Faculdade de Medicina Veterináriae Zootecnia da Universidade de São Paulo, São Paulo, SP, Brazil. Electronic address: lfelicio@usp.br.
Abstract
AIMS: Prolactin is a major immunomodulator. The present study evaluated the effects of short-term hyperprolactinemia induced by domperidone before ovalbumin antigenic challenge on the lung's allergic inflammatory response. MAIN METHODS: To induce hyperprolactinemia, domperidone was injected in rats at a dose of 5.1mg·kg(-1) per day, i.p., for 5days from 10th to 14th day after OVA immunization. Total and differential leukocyte counts from bronchoalveolar lavage (BAL), femoral marrow lavage (FML), and blood were analyzed. The percentages of mucus and collagen production were evaluated. Levels of corticosterone and prolactin in serum, interleukin-4 (IL-4), IL-6, IL-10, tumor necrosis factor α (TNF-α) in lung explants supernatants were measured and interferon gamma (IFN-γ) in bronchiolar lavage cells suspensions (BAL) was measured. KEY FINDINGS: The rats that were subjected to short-term hyperprolactinemia exhibited a decrease in leukocyte counts in bronchoalveolar lavage, cellularity decrease in femoral marrow lavage fluid, a lower percentage of mucus, and an increase in lung IL-4, IL-6, IL-10, TNF-α and IFN-γ expression. SIGNIFICANCE: Hyperprolactinemia induced before antigenic challenge decreased allergic lung inflammation. These data suggest that prolactin may play a role in the pathophysiology of asthma. The present study demonstrates a prospective beneficial side effect of domperidone for asthmatic patients.
AIMS: Prolactin is a major immunomodulator. The present study evaluated the effects of short-term hyperprolactinemia induced by domperidone before ovalbumin antigenic challenge on the lung's allergic inflammatory response. MAIN METHODS: To induce hyperprolactinemia, domperidone was injected in rats at a dose of 5.1mg·kg(-1) per day, i.p., for 5days from 10th to 14th day after OVA immunization. Total and differential leukocyte counts from bronchoalveolar lavage (BAL), femoral marrow lavage (FML), and blood were analyzed. The percentages of mucus and collagen production were evaluated. Levels of corticosterone and prolactin in serum, interleukin-4 (IL-4), IL-6, IL-10, tumor necrosis factor α (TNF-α) in lung explants supernatants were measured and interferon gamma (IFN-γ) in bronchiolar lavage cells suspensions (BAL) was measured. KEY FINDINGS: The rats that were subjected to short-term hyperprolactinemia exhibited a decrease in leukocyte counts in bronchoalveolar lavage, cellularity decrease in femoral marrow lavage fluid, a lower percentage of mucus, and an increase in lung IL-4, IL-6, IL-10, TNF-α and IFN-γ expression. SIGNIFICANCE: Hyperprolactinemia induced before antigenic challenge decreased allergic lung inflammation. These data suggest that prolactin may play a role in the pathophysiology of asthma. The present study demonstrates a prospective beneficial side effect of domperidone for asthmatic patients.
Authors: Carmen Clapp; Norma Adán; María G Ledesma-Colunga; Mariana Solís-Gutiérrez; Jakob Triebel; Gonzalo Martínez de la Escalera Journal: Cell Mol Life Sci Date: 2016-03-29 Impact factor: 9.261