Yaoyang Liu1, Zhiguo Zhang1, Qianmei Jin1, Yang Liu2, Zijian Kang1, Yongbao Huo1, Zhengwen He3, Xu Feng4, Jian Yin1, Xin Wu1, Huaizhou Wang5, Huji Xu6,7,8. 1. Department of Rheumatology and Immunology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China. 2. Department of Cardiovascular Surgery, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, China. 3. Department of Laboratory Diagnosis, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, China. 4. Department of Reproductive Medical Center, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China. 5. Department of Laboratory Diagnosis, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, China. whz_sh@163.com. 6. Department of Rheumatology and Immunology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China. xuhuji@smmu.edu.cn. 7. Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China. xuhuji@smmu.edu.cn. 8. Peking-Tsinghua Center for Life Sciences, Tsinghua University, Beijing, China. xuhuji@smmu.edu.cn.
Abstract
PURPOSE: Hyperprolactinemia (HPRL) has been reported in many autoimmune diseases. However, the serum autoantibody profile and peripheral B-cell subset distribution in women with HPRL are largely unknown. The current study aimed to investigate the autoantibody prevalence and cytokine levels as well as to further explore the B-cell subset distribution in women with HPRL. METHODS: Sera from 202 women with HPRL and 97 healthy women were included in this study. All sera were examined for prolactin (PRL), anti-nuclear antibody (ANA), rheumatoid factor, anticardiolipin (ACL), immunoglobulin G, immunoglobulin M, complement 3, complement 4, interleukin 4 (IL-4) and interleukin 6 (IL-6). Peripheral blood was collected from 22 women with HPRL and 19 healthy women, and B-cell subsets were measured by flow cytometry. RESULTS: At least one autoantibody was found in 47 out of 202 women with HPRL compared with 9 of 97 healthy women (p < 0.001). The levels of IL-4 (p < 0.0001) and IL-6 (p < 0.0001) were significantly higher in women with HPRL than in healthy women. The percentages of naive IgD+IgM- B cells (BND cells, p < 0.0001), antibody-secreting cells (p = 0.007) and unswitched memory B cells (p = 0.004) among the total B cells from HPRL women were significantly higher than those from healthy women. CONCLUSIONS: Women with HPRL had a higher prevalence of autoantibodies, higher serum levels of IL-4 and IL-6, and more BND cells, antibody-secreting B cells and unswitched memory B cells than healthy women. These data imply that a high level of PRL is associated with autoimmune diseases.
PURPOSE:Hyperprolactinemia (HPRL) has been reported in many autoimmune diseases. However, the serum autoantibody profile and peripheral B-cell subset distribution in women with HPRL are largely unknown. The current study aimed to investigate the autoantibody prevalence and cytokine levels as well as to further explore the B-cell subset distribution in women with HPRL. METHODS: Sera from 202 women with HPRL and 97 healthy women were included in this study. All sera were examined for prolactin (PRL), anti-nuclear antibody (ANA), rheumatoid factor, anticardiolipin (ACL), immunoglobulin G, immunoglobulin M, complement 3, complement 4, interleukin 4 (IL-4) and interleukin 6 (IL-6). Peripheral blood was collected from 22 women with HPRL and 19 healthy women, and B-cell subsets were measured by flow cytometry. RESULTS: At least one autoantibody was found in 47 out of 202 women with HPRL compared with 9 of 97 healthy women (p < 0.001). The levels of IL-4 (p < 0.0001) and IL-6 (p < 0.0001) were significantly higher in women with HPRL than in healthy women. The percentages of naive IgD+IgM- B cells (BND cells, p < 0.0001), antibody-secreting cells (p = 0.007) and unswitched memory B cells (p = 0.004) among the total B cells from HPRL women were significantly higher than those from healthy women. CONCLUSIONS:Women with HPRL had a higher prevalence of autoantibodies, higher serum levels of IL-4 and IL-6, and more BND cells, antibody-secreting B cells and unswitched memory B cells than healthy women. These data imply that a high level of PRL is associated with autoimmune diseases.
Authors: I Krause; Z Blumenfeld; M Malchinsky; S Cohen; M Blank; A Eldor; B Weksler; K Schweitzer; Y Shoenfeld Journal: Lupus Date: 1998 Impact factor: 2.911
Authors: Christopher M Tipton; Christopher F Fucile; Jaime Darce; Asiya Chida; Travis Ichikawa; Ivan Gregoretti; Sandra Schieferl; Jennifer Hom; Scott Jenks; Ron J Feldman; Ramit Mehr; Chungwen Wei; F Eun-Hyung Lee; Wan Cheung Cheung; Alexander F Rosenberg; Iñaki Sanz Journal: Nat Immunol Date: 2015-05-25 Impact factor: 25.606
Authors: J Andrew Duty; Peter Szodoray; Nai-Ying Zheng; Kristi A Koelsch; Qingzhao Zhang; Mike Swiatkowski; Melissa Mathias; Lori Garman; Christina Helms; Britt Nakken; Kenneth Smith; A Darise Farris; Patrick C Wilson Journal: J Exp Med Date: 2008-12-22 Impact factor: 14.307