| Literature DB >> 26477248 |
Christian Altenbach1, Carlos J López1, Kálmán Hideg2, Wayne L Hubbell3.
Abstract
Structural and dynamical characterization of proteins is of central importance in understanding the mechanisms underlying their biological functions. Site-directed spin labeling (SDSL) combined with continuous-wave electron paramagnetic resonance (CW EPR) spectroscopy has shown the capability of providing this information with site-specific resolution under physiological conditions for proteins of any degree of complexity, including those associated with membranes. This chapter introduces methods commonly employed for SDSL and describes selected CW EPR-based methods that can be applied to (1) map secondary and tertiary protein structure, (2) determine membrane protein topology, (3) measure protein backbone flexibility, and (4) reveal the existence of conformational exchange at equilibrium.Entities:
Keywords: CW EPR; Distance measurements; EPR lineshape analysis; Nitroxide scanning; Protein dynamics; Protein structure; Site-directed spin labeling; Solvent accessibility; Spin interaction
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Year: 2015 PMID： 26477248 DOI： 10.1016/bs.mie.2015.08.006
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600