Hee Joung Kim1, Seung Hun Jang2, Jeong-Seon Ryu3, Jeong Eun Lee4, Young Chul Kim5, Min Ki Lee6, Tae Won Jang7, Shin-Yup Lee8, Hidehiro Nakamura9, Natsumi Nishikata9, Maiko Mori9, Yasushi Noguchi9, Hiroshi Miyano9, Kye Young Lee10. 1. Department of Internal Medicine, Konkuk University School of Medicine, Seoul, Republic of Korea. 2. Department of Internal Medicine, Hallym University Sacred Heart Hospital, Anyang, Republic of Korea. 3. Department of Internal Medicine, Inha University Hospital, Incheon, Republic of Korea. 4. Department of Internal Medicine, Chungnam National University Hospital and Cancer Research Institute, Deajon, Republic of Korea. 5. Department of Internal Medicine, Chonnam National University Medical School, Hwasun Hospital, Hwasun, Republic of Korea. 6. Department of Internal Medicine, Pusan National University Hospital, Pusan, Republic of Korea. 7. Department of Internal Medicine, Kosin University Medical College, Pusan, Republic of Korea. 8. Department of Internal Medicine, Kyungpook National University Hospital, Daegu, Republic of Korea. 9. Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan. 10. Department of Internal Medicine, Konkuk University School of Medicine, Seoul, Republic of Korea. Electronic address: kyleemd@kuh.ac.kr.
Abstract
INTRODUCTION: Previous studies have shown that plasma free amino acid (PFAA) profiles are altered in cancer patients compared with healthy controls. A multivariate index based on PFAAs was generated from a Japanese dataset and has been previously demonstrated to be clinically valuable for discriminating patients in the early stages of lung cancer. However, it remains unclear whether similar PFAA profile changes occur in cancer patients from other populations. Therefore, this study aimed to validate the performance of this index in discriminating lung cancer patients from controls in the Korean population. METHODS: Samples were collected from a total of 142 Korean subjects (72 lung cancer/70 controls) for this study. PFAAs were quantified by high-performance liquid chromatography-electrospray ionization-mass spectrometry, and the clinical performance characteristics of the amino acid multivariate index were evaluated across cancer stages and histological types. RESULTS: The concentrations of several PFAAs were significantly decreased in the Korean lung cancer patients compared with the controls. Significant decreases in threonine, citrulline, histidine and tryptophan and increases in proline, isoleucine, phenylalanine and ornithine were observed, which are similar to the PFAA changes reported by a previous Japanese study. The area under the receiver-operator characteristic curve (AUC of the ROC) for the index was 0.80, and similar performances were demonstrated for the different histological types. CONCLUSIONS: These results suggest that the amino acid multivariate index previously developed from a Japanese dataset has the potential to aid in the early detection of lung cancers of different histological types in Korean patients.
INTRODUCTION: Previous studies have shown that plasma free amino acid (PFAA) profiles are altered in cancerpatients compared with healthy controls. A multivariate index based on PFAAs was generated from a Japanese dataset and has been previously demonstrated to be clinically valuable for discriminating patients in the early stages of lung cancer. However, it remains unclear whether similar PFAA profile changes occur in cancerpatients from other populations. Therefore, this study aimed to validate the performance of this index in discriminating lung cancerpatients from controls in the Korean population. METHODS: Samples were collected from a total of 142 Korean subjects (72 lung cancer/70 controls) for this study. PFAAs were quantified by high-performance liquid chromatography-electrospray ionization-mass spectrometry, and the clinical performance characteristics of the amino acid multivariate index were evaluated across cancer stages and histological types. RESULTS: The concentrations of several PFAAs were significantly decreased in the Korean lung cancerpatients compared with the controls. Significant decreases in threonine, citrulline, histidine and tryptophan and increases in proline, isoleucine, phenylalanine and ornithine were observed, which are similar to the PFAA changes reported by a previous Japanese study. The area under the receiver-operator characteristic curve (AUC of the ROC) for the index was 0.80, and similar performances were demonstrated for the different histological types. CONCLUSIONS: These results suggest that the amino acid multivariate index previously developed from a Japanese dataset has the potential to aid in the early detection of lung cancers of different histological types in Korean patients.
Authors: Francis Berthias; Belkis Maatoug; Gary L Glish; Fathi Moussa; Philippe Maitre Journal: J Am Soc Mass Spectrom Date: 2018-02-21 Impact factor: 3.109