| Literature DB >> 26476216 |
Hongpeng He1, Dandan Wang1, Hailin Yao1, Zhaoqiang Wei1, Yongwei Lai1, Juan Hu1, Xuena Liu1, Yijie Wang1, Hao Zhou1, Nan Wang1, Xue-Gang Luo1, Tong-Cun Zhang2.
Abstract
The transcriptional coactivator p300 is highly expressed in breast cancer tissues. MRTF-A is a transcription factor governed by the Rho-GTPase-actin signaling pathway. The purpose of this study was to explore the role of p300 in breast cancer metastasis. Here we showed that the motility of breast cancer cells was enhanced by the overexpression of p300, meanwhile, the transcription of migration-related genes was upregulated. Depletion of p300 downregulated the migration-related genes and slowed down the migration of breast cancer cells. p300 worked synergistically with MRTF-A to activate the transcription of MYH9, MYL9 and CYR61. As identified by co-IP, p300 interacted with the C-terminal TAD domain of MRTF-A. And together with MRTF-A, p300 was associated with the target gene promoters. Furthermore, MRTF-A was found to be acetylated in MCF-7 breast cancer cells. These results demonstrated the involvement of p300 in the MRTF-A mediated gene regulation and breast cancer cell migration.Entities:
Keywords: Breast cancer; Cell migration; MRTF-A; Transcription; p300
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Year: 2015 PMID： 26476216 DOI： 10.1016/j.bbrc.2015.10.060
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575