Myoblast cell sheet transplantation enhances the endogenous regenerative abilities of infant hearts in rats with myocardial infarction.

The shortage of heart transplantation donors for infants is severe. Regenerative cell therapy has been expected to offer new methods of treatment, and this study was about regenerative cell therapy for infant hearts. The aims of the present study were to clarify the effects of regenerative cell therapy on the infant heart. The heart impairment model and tissue-engineered myoblast cell sheets were used for regenerative cell therapy. Infant rats (n = 54) aged 2 weeks and adult rats aged 12 weeks (n = 35) were used. Myocardial infarction (MI) was induced as the heart impairment model and triple-layer myoblast cell sheets were used for transplantation to MI lesions. Infant rats after MI had better self-regenerative ability in wall thickness, fibrosis and cardiac function and we observed greater numbers of proliferating cardiomyocytes than in adults. Moreover, infant MI rats treated with myoblast cell sheets showed better results in wall thickness, fibrosis and cardiac function than infant MI rats without myoblast cell sheets, because of the positive effect that myoblast cell sheets had on proliferating cardiomyocytes, increasing vascular networks and accumulating c-kit-positive cells. We clarified that regenerative cell therapy enhances the endogenous regenerative ability of infant hearts in rats with MI; moreover, it has a greater therapeutic effect on infant hearts than on adult hearts, because of the ability of infant hearts for cardiomyocyte proliferation. The present paper provides essential new data for clinical therapy in infant patients.