Literature DB >> 26455965

Hepatic proteomic analysis revealed altered metabolic pathways in insulin resistant Akt1(+/-)/Akt2(-/-) mice.

Brian A Pedersen1, Weiwen Wang2, Jared F Taylor1, Omar S Khattab3, Yu-Han Chen4, Robert A Edwards5, Puya G Yazdi1, Ping H Wang6.   

Abstract

OBJECTIVE: The aim of this study was to identify liver proteome changes in a mouse model of severe insulin resistance and markedly decreased leptin levels.
METHODS: Two-dimensional differential gel electrophoresis was utilized to identify liver proteome changes in AKT1(+/-)/AKT2(-/-) mice. Proteins with altered levels were identified with tandem mass spectrometry. Ingenuity Pathway Analysis was performed for the interpretation of the biological significance of the observed proteomic changes.
RESULTS: 11 proteins were identified from 2 biological replicates to be differentially expressed by a ratio of at least 1.3 between age-matched insulin resistant (Akt1(+/-)/Akt2(-/-)) and wild type mice. Albumin and mitochondrial ornithine aminotransferase were detected from multiple spots, which suggest post-translational modifications. Enzymes of the urea cycle were common members of top regulated pathways.
CONCLUSION: Our results help to unveil the regulation of the liver proteome underlying altered metabolism in an animal model of severe insulin resistance.
Copyright © 2015 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  AKT; Insulin resistance; Liver; Mouse; Proteomics

Mesh：

Substances：

Year:  2015        PMID： 26455965      PMCID： PMC4641788          DOI： 10.1016/j.metabol.2015.09.008

Source DB:  PubMed          Journal:  Metabolism        ISSN： 0026-0495            Impact factor:   8.694


  67 in total

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