Literature DB >> 26453947

Fluorescent labeling reliably identifies Chlamydia trachomatis in living human endometrial cells and rapidly and accurately quantifies chlamydial inclusion forming units.

Rodolfo D Vicetti Miguel1, Kevin J Henschel2, Fiorela C Dueñas Lopez2, Nirk E Quispe Calla2, Thomas L Cherpes3.   

Abstract

Chlamydia replication requires host lipid acquisition, allowing flow cytometry to identify Chlamydia-infected cells that accumulated fluorescent Golgi-specific lipid. Herein, we describe modifications to currently available methods that allow precise differentiation between uninfected and Chlamydia trachomatis-infected human endometrial cells and rapidly and accurately quantify chlamydial inclusion forming units.
Copyright © 2015. Published by Elsevier B.V.

Entities:  

Keywords:  Ceramide; Chlamydia trachomatis; Flow cytometry; Human endometrial cells; Inclusion forming units

Mesh：

Substances：

Year:  2015        PMID： 26453947      PMCID： PMC4993108          DOI： 10.1016/j.mimet.2015.10.003

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  10 in total

1.  Fluorescent labelling of intracellular bacteria in living host cells.

Authors:  H Boleti; D M Ojcius; A Dautry-Varsat
Journal:  J Microbiol Methods       Date:  2000-05       Impact factor: 2.363

2.  Clonal isolation of chlamydia-infected cells using flow cytometry.

Authors:  Damir T Alzhanov; Robert J Suchland; Antony C Bakke; Walter E Stamm; Daniel D Rockey
Journal:  J Microbiol Methods       Date:  2006-09-25       Impact factor: 2.363

3.  NIH Image to ImageJ: 25 years of image analysis.

Authors:  Caroline A Schneider; Wayne S Rasband; Kevin W Eliceiri
Journal:  Nat Methods       Date:  2012-07       Impact factor: 28.547

4.  Sphingomyelin trafficking in Chlamydia pneumoniae-infected cells.

Authors:  K Wolf; T Hackstadt
Journal:  Cell Microbiol       Date:  2001-03       Impact factor: 3.715

5.  Endometrial leukocyte subpopulations associated with Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis genital tract infection.

Authors:  Seth D Reighard; Richard L Sweet; Claudia Vicetti Miguel; Rodolfo D Vicetti Miguel; Mamatha Chivukula; Uma Krishnamurti; Thomas L Cherpes
Journal:  Am J Obstet Gynecol       Date:  2011-05-20       Impact factor: 8.661

6.  Lipid metabolism in Chlamydia trachomatis-infected cells: directed trafficking of Golgi-derived sphingolipids to the chlamydial inclusion.

Authors:  T Hackstadt; M A Scidmore; D D Rockey
Journal:  Proc Natl Acad Sci U S A       Date:  1995-05-23       Impact factor: 11.205

7.  Cultivation and Laboratory Maintenance of Chlamydia trachomatis.

Authors:  Marci A Scidmore
Journal:  Curr Protoc Microbiol       Date:  2005-07

8.  Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction.

Authors:  Dagmar Heuer; Anette Rejman Lipinski; Nikolaus Machuy; Alexander Karlas; Andrea Wehrens; Frank Siedler; Volker Brinkmann; Thomas F Meyer
Journal:  Nature       Date:  2008-12-07       Impact factor: 49.962

9.  CPAF: a Chlamydial protease in search of an authentic substrate.

Authors:  Allan L Chen; Kirsten A Johnson; Jennifer K Lee; Christine Sütterlin; Ming Tan
Journal:  PLoS Pathog       Date:  2012-08-02       Impact factor: 6.823

10.  Chlamydia trachomatis infection control programs: lessons learned and implications for vaccine development.

Authors:  Jean M Chavez; Rodolfo D Vicetti Miguel; Thomas L Cherpes
Journal:  Infect Dis Obstet Gynecol       Date:  2011-11-14
  10 in total
  3 in total

1.  IL-4-secreting eosinophils promote endometrial stromal cell proliferation and prevent Chlamydia-induced upper genital tract damage.

Authors:  Rodolfo D Vicetti Miguel; Nirk E Quispe Calla; Darlene Dixon; Robert A Foster; Andrea Gambotto; Stephen D Pavelko; Luanne Hall-Stoodley; Thomas L Cherpes
Journal:  Proc Natl Acad Sci U S A       Date:  2017-08-01       Impact factor: 11.205

2.  Intravaginal Chlamydia trachomatis Challenge Infection Elicits TH1 and TH17 Immune Responses in Mice That Promote Pathogen Clearance and Genital Tract Damage.

Authors:  Rodolfo D Vicetti Miguel; Nirk E Quispe Calla; Stephen D Pavelko; Thomas L Cherpes
Journal:  PLoS One       Date:  2016-09-08       Impact factor: 3.240

3.  Dendritic cell function and pathogen-specific T cell immunity are inhibited in mice administered levonorgestrel prior to intranasal Chlamydia trachomatis infection.

Authors:  Nirk E Quispe Calla; Rodolfo D Vicetti Miguel; Ao Mei; Shumin Fan; Jocelyn R Gilmore; Thomas L Cherpes
Journal:  Sci Rep       Date:  2016-11-28       Impact factor: 4.379
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.