David Johannes Scholz1, Angela M Otto1, Josef Hintermair1, Franz Schilling1,2, Annette Frank2, Ulrich Köllisch1, Martin A Janich3, Rolf F Schulte3, Markus Schwaiger2, Axel Haase1, Marion I Menzel4. 1. Institute of Medical Engineering, Technische Universität München, Munich, Germany. 2. Nuklearmedizinische Klinik und Poliklinik, Klinikum rechts der Isar, Technische Universität München, Munich, Germany. 3. Institute of Medical Engineering, General Electric Global Research, Freisinger Landstraße 50, 85748, Garching b. Munich, Germany. 4. Institute of Medical Engineering, General Electric Global Research, Freisinger Landstraße 50, 85748, Garching b. Munich, Germany. menzel@ge.com.
Abstract
OBJECTIVE: (13)C metabolic MRI using hyperpolarized (13)C-bicarbonate enables preclinical detection of pH. To improve signal-to-noise ratio, experimental procedures were refined, and the influence of pH, buffer capacity, temperature, and field strength were investigated. MATERIALS AND METHODS: Bicarbonate preparation was investigated. Bicarbonate was prepared and applied in spectroscopy at 1, 3, 14 T using pure dissolution, culture medium, and MCF-7 cell spheroids. Healthy rats were imaged by spectral-spatial spiral acquisition for spatial and temporal bicarbonate distribution, pH mapping, and signal decay analysis. RESULTS: An optimized preparation technique for maximum solubility of 6 mol/L and polarization levels of 19-21% is presented; T1 and SNR dependency on field strength, buffer capacity, and pH was investigated. pH mapping in vivo is demonstrated. CONCLUSION: An optimized bicarbonate preparation and experimental procedure provided improved T1 and SNR values, allowing in vitro and in vivo applications.
OBJECTIVE: (13)C metabolic MRI using hyperpolarized (13)C-bicarbonate enables preclinical detection of pH. To improve signal-to-noise ratio, experimental procedures were refined, and the influence of pH, buffer capacity, temperature, and field strength were investigated. MATERIALS AND METHODS:Bicarbonate preparation was investigated. Bicarbonate was prepared and applied in spectroscopy at 1, 3, 14 T using pure dissolution, culture medium, and MCF-7 cell spheroids. Healthy rats were imaged by spectral-spatial spiral acquisition for spatial and temporal bicarbonate distribution, pH mapping, and signal decay analysis. RESULTS: An optimized preparation technique for maximum solubility of 6 mol/L and polarization levels of 19-21% is presented; T1 and SNR dependency on field strength, buffer capacity, and pH was investigated. pH mapping in vivo is demonstrated. CONCLUSION: An optimized bicarbonate preparation and experimental procedure provided improved T1 and SNR values, allowing in vitro and in vivo applications.
Authors: David Johannes Scholz; Martin A Janich; Ulrich Köllisch; Rolf F Schulte; Jan H Ardenkjaer-Larsen; Annette Frank; Axel Haase; Markus Schwaiger; Marion I Menzel Journal: Magn Reson Med Date: 2014-07-15 Impact factor: 4.668
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