Francisco J Manzano-Moreno1, Rosa Medina-Huertas2, Javier Ramos-Torrecillas3, Olga García-Martínez3, Concepción Ruiz4. 1. Department of Stomatology (Head: Prof. Pilar Aranda-Ramirez), School of Dentistry, University of Granada, Spain; Biomedical Group (BIO277), Department of Nursing (Head: Prof. Pilar Aranda-Ramirez), Faculty of Health Sciences, University of Granada, Spain; Instituto Investigación Biosanitaria, ibs.Granada (Head: Prof. Nicolas Olea-Serrano), Spain. 2. Biomedical Group (BIO277), Department of Nursing (Head: Prof. Pilar Aranda-Ramirez), Faculty of Health Sciences, University of Granada, Spain. 3. Biomedical Group (BIO277), Department of Nursing (Head: Prof. Pilar Aranda-Ramirez), Faculty of Health Sciences, University of Granada, Spain; Instituto Investigación Biosanitaria, ibs.Granada (Head: Prof. Nicolas Olea-Serrano), Spain. 4. Biomedical Group (BIO277), Department of Nursing (Head: Prof. Pilar Aranda-Ramirez), Faculty of Health Sciences, University of Granada, Spain; Instituto Investigación Biosanitaria, ibs.Granada (Head: Prof. Nicolas Olea-Serrano), Spain; Institute of Neuroscience (Head: Prof. Pilar Aranda-Ramirez), Parque Tecnológico Ciencias de la Salud, Armilla (Granada), University of Granada, Spain. Electronic address: crr@ugr.es.
Abstract
OBJECTIVES: The objective of this study was to determine the effect of LLDL therapy on the gene expression of osteoblast markers of growth and differentiation. MATERIALS AND METHODS: The MG-63 cell line was exposed to diode laser (ezLase) of 940 nm at 1-1.5 W and 3-4 J, and gene expressions (Runx-2, alkaline phosphatase [ALP], type I collagen [Col-I], osterix [OSX], osteocalcin [OSC], osteoprotegerin [OPG], bone morphogenetic protein [BMP]-2 and -7, transforming growth factor-β1 [TGF-β1], and TGF-β receptors [TGF-β R1, TGF-β R2; TGF-β R3]) were evaluated by quantitative RT-PCR. RESULTS: LLDL treatment stimulated the expression of osteoblast differentiation markers ALP, Col-I, Runx-2, and OSX in relation to the doses applied (P < 0.05), but no changes were detected in OSC, OPG, or BMP-7 at any study dose. This effect may be mediated by TGF-β1 and BMP-2, given that the treatment increased their expression and that of TGF-β receptors R1, R2, and R3 (P < 0.001). CONCLUSION: These results suggest that the biostimulatory effect of laser therapy on osteoblasts may be attributable to the release of autocrine factors in response to the irradiation. A clinical trial is warranted to test its therapeutic usefulness in bone tissue regeneration and to define a treatment protocol.
OBJECTIVES: The objective of this study was to determine the effect of LLDL therapy on the gene expression of osteoblast markers of growth and differentiation. MATERIALS AND METHODS: The MG-63 cell line was exposed to diode laser (ezLase) of 940 nm at 1-1.5 W and 3-4 J, and gene expressions (Runx-2, alkaline phosphatase [ALP], type I collagen [Col-I], osterix [OSX], osteocalcin [OSC], osteoprotegerin [OPG], bone morphogenetic protein [BMP]-2 and -7, transforming growth factor-β1 [TGF-β1], and TGF-β receptors [TGF-β R1, TGF-β R2; TGF-β R3]) were evaluated by quantitative RT-PCR. RESULTS: LLDL treatment stimulated the expression of osteoblast differentiation markers ALP, Col-I, Runx-2, and OSX in relation to the doses applied (P < 0.05), but no changes were detected in OSC, OPG, or BMP-7 at any study dose. This effect may be mediated by TGF-β1 and BMP-2, given that the treatment increased their expression and that of TGF-β receptors R1, R2, and R3 (P < 0.001). CONCLUSION: These results suggest that the biostimulatory effect of laser therapy on osteoblasts may be attributable to the release of autocrine factors in response to the irradiation. A clinical trial is warranted to test its therapeutic usefulness in bone tissue regeneration and to define a treatment protocol.
Authors: M Paschalidou; E Athanasiadou; K Arapostathis; N Kotsanos; P T Koidis; Athina Bakopoulou; Anna Theocharidou Journal: Clin Oral Investig Date: 2019-05-08 Impact factor: 3.573
Authors: Francisco Javier Manzano-Moreno; Javier Ramos-Torrecillas; Lucia Melguizo-Rodríguez; Rebeca Illescas-Montes; Concepción Ruiz; Olga García-Martínez Journal: Int J Med Sci Date: 2018-02-12 Impact factor: 3.738