| Literature DB >> 26443211 |
David G Ousterout1, Charles A Gersbach2,3,4.
Abstract
The development of a facile genome engineering technology based on transcription activator-like effector nucleases (TALENs) has led to significant advances in diverse areas of science and medicine. In this review, we provide a broad overview of the development of TALENs and the use of this technology in basic science, biotechnology, and biomedical applications. This includes the discovery of DNA recognition by TALEs, engineering new TALE proteins to diverse targets, general advances in nuclease-based editing strategies, and challenges that are specific to various applications of the TALEN technology. We review examples of applying TALENs for studying gene function and regulation, generating disease models, and developing gene therapies. The current status of genome editing and future directions for other uses of these technologies are also discussed.Entities:
Keywords: Gene editing; Gene targeting; Gene therapy; Genome engineering
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Year: 2016 PMID： 26443211 PMCID： PMC5316914 DOI： 10.1007/978-1-4939-2932-0_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745