Jazmín Hernández-Montoya1, Julia Pérez-Ramos2, Martha Montaño3, Alejandra Ramírez-Venegas4, Raúl H Sansores4, Gloria Pérez-Rubio5, Mónica Velázquez-Uncal4, Angel Camarena5, Carlos Ramos3, Ramcés Falfán-Valencia5. 1. Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Xochimilco-Iztapalapa-Cuajimalpa, México DF, México. 2. Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, México DF, México. 3. Departamento de investigación en fibrosis pulmonar, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas (INER), México DF, México. 4. Departamento de Investigación en Tabaquismo y EPOC, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas (INER), México DF, México. 5. Laboratorio HLA, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas (INER), México DF, México.
Abstract
AIM: To evaluate association of single nucleotide polymorphisms (SNPs) in the MMP1, MMP2, MMP9 and MMP12 genes and serum MMP-2 and MMP-9 levels in smoking chronic obstructive pulmonary disease (COPD) patients. MATERIALS & METHODS: Genotyping using real-time PCR in 330 smokers with COPD (COPD), 658 smokers without COPD (SNC) and 150 nonsmokers (NCNS), the analysis of samples used was χ(2) test. Using ELISA, the proteins were evaluated. Multiple comparisons were made by ANOVA. RESULTS: rs243864 (OR: 7.44; 95% CI: 3.62-15.26) and rs11646643 (OR: 1.58; 95% CI: 1.07-2.34) of the MMP-2 gene and rs3918253 (OR: 1.72; 95% CI: 1.08-2.71) of the MMP-9 gene, were associated with the risk of COPD. Serum MMP-2 level in the COPD group was lower compared with SNC (p < 0.05). Serum MMP-9 level was elevated in the COPD group compared with SNC (p < 0.05). CONCLUSION: Polymorphisms in MMP2 and MMP9 but not in MMP1 and MMP12 are associated with the risk of COPD in the Mexican mestizo population.
AIM: To evaluate association of single nucleotide polymorphisms (SNPs) in the MMP1, MMP2, MMP9 and MMP12 genes and serum MMP-2 and MMP-9 levels in smoking chronic obstructive pulmonary disease (COPD) patients. MATERIALS & METHODS: Genotyping using real-time PCR in 330 smokers with COPD (COPD), 658 smokers without COPD (SNC) and 150 nonsmokers (NCNS), the analysis of samples used was χ(2) test. Using ELISA, the proteins were evaluated. Multiple comparisons were made by ANOVA. RESULTS:rs243864 (OR: 7.44; 95% CI: 3.62-15.26) and rs11646643 (OR: 1.58; 95% CI: 1.07-2.34) of the MMP-2 gene and rs3918253 (OR: 1.72; 95% CI: 1.08-2.71) of the MMP-9 gene, were associated with the risk of COPD. Serum MMP-2 level in the COPD group was lower compared with SNC (p < 0.05). Serum MMP-9 level was elevated in the COPD group compared with SNC (p < 0.05). CONCLUSION: Polymorphisms in MMP2 and MMP9 but not in MMP1 and MMP12 are associated with the risk of COPD in the Mexican mestizo population.
Authors: Murali M S Balla; Sejal Desai; Pallavi Purwar; Amit Kumar; Prashant Bhandarkar; Yogesh K Shejul; C S Pramesh; S Laskar; Badri N Pandey Journal: Sci Rep Date: 2016-11-04 Impact factor: 4.379