| Literature DB >> 26434279 |
V P Veerapur1, K R Prabhakar2, B S Thippeswamy3, Punit Bansal2, K K Srinivasan2, M K Unnikrishnan2.
Abstract
The present study was designed to investigate the effects of the ethanol extract of Ficus racemosa (FRE) on biochemical parameters in type 2-like diabetes, induced by a combination of standardised high-fat diet and low-dose streptozotocin (25mgkg(-1), i.p.) in rats. To elucidate the mode of action of FRE, its effects on a battery of targets involved in glucose homeostasis was evaluated. FRE (200 and 400mgkg(-1), p.o.), in a dose-dependent manner, altered the biochemical parameters and significantly improved glucose tolerance and HDL-c levels. In different bioassays, FRE showed inhibition of PTP-1B (IC50 12.1μg/mL) and DPP-IV (42.5%). FRE exhibited 82.6% binding to PPAR-γ. Furthermore FRE exhibited stimulation of glucose uptake by skeletal muscles (hemi-diaphragm). Bergenin was quantified in bioactive-FRE by high-performance liquid chromatography (0.15%w/w). This is the first report demonstrating the effectiveness of F. racemosa stem bark in type 2 diabetes and targets involved in it.Entities:
Keywords: Bergenin; DPP-IV; Ficus racemosa; Glucose uptake; High-fat diet-fed; PPAR-γ; PTB-1B; STZ
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Year: 2011 PMID： 26434279 DOI： 10.1016/j.foodchem.2011.10.052
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514