Literature DB >> 26432950

Establishment and validation of a 384-well antibacterial assay amenable for high-throughput screening and combination testing.

Arti Mishra1, Svetlana V Dobritsa2, Marie-Laure Crouch3, John Rabenstein3, Joycelyn Xiang Yi Lee1, Saravanakumar Dhakshinamoorthy4.   

Abstract

A 384-well-based antibacterial assay amenable for high-throughput screening and combination testing is described. The assay uses 100-500nL of test compounds and tolerates up to 2.5% dimethyl sulfoxide concentrations. It can be used for screening compound libraries and testing combinatory/synergistic/antagonistic effects of antibiotics, small molecules, and natural product extracts.
Copyright © 2015 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Antibacterial assay; Combination testing; DMSO tolerance; HTS

Mesh：

Substances：

Year:  2015        PMID： 26432950     DOI： 10.1016/j.mimet.2015.09.019

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  4 in total

1.  Medium-Throughput Screen of Microbially Produced Serotonin via a G-Protein-Coupled Receptor-Based Sensor.

Authors:  Amy M Ehrenworth; Tauris Claiborne; Pamela Peralta-Yahya
Journal:  Biochemistry       Date:  2017-08-28       Impact factor: 3.162

2.  Optimization of a High-Throughput 384-Well Plate-Based Screening Platform with Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 15442 Biofilms.

Authors:  Shella Gilbert-Girard; Kirsi Savijoki; Jari Yli-Kauhaluoma; Adyary Fallarero
Journal:  Int J Mol Sci       Date:  2020-04-25       Impact factor: 5.923

3.  Novel Anti-Campylobacter Compounds Identified Using High Throughput Screening of a Pre-selected Enriched Small Molecules Library.

Authors:  Anand Kumar; Mary Drozd; Ruby Pina-Mimbela; Xiulan Xu; Yosra A Helmy; Janet Antwi; James R Fuchs; Corey Nislow; Jillian Templeton; Patrick J Blackall; Gireesh Rajashekara
Journal:  Front Microbiol       Date:  2016-04-06       Impact factor: 5.640

4.  Identification of Three Antimicrobials Activating Serotonin Receptor 4 in Colon Cells.

Authors:  Emily A Yasi; Aurelia A Allen; Widianti Sugianto; Pamela Peralta-Yahya
Journal:  ACS Synth Biol       Date:  2019-11-12       Impact factor: 5.110
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.