Literature DB >> 2641485

ELISA with enzyme amplification for sensitive detection of staphylococcal enterotoxins in food.

H Windemann1, J Lüthy, M Maurer.   

Abstract

A highly sensitive amplified ELISA in microtitre plates for the detection of staphylococcal enterotoxins compared favourably with a commercially available kit. The amplified ELISA demonstrated a reduced effect of the food sample matrix and an ease of handling. Using the amplified ELISA, the influence of different parameters (pH, time, temperature, re-extraction, concentration procedure) on the recovery efficiency and assay reliability was tested on foods implicated in food poisoning outbreaks as well as on spiked foods containing low levels of enterotoxins.

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Year:  1989        PMID： 2641485     DOI： 10.1016/0168-1605(89)90077-9

Source DB:  PubMed          Journal:  Int J Food Microbiol        ISSN： 0168-1605            Impact factor:   5.277


  2 in total

1.  Detection of enterotoxigenic Staphylococcus aureus in dried skimmed milk: use of the polymerase chain reaction for amplification and detection of staphylococcal enterotoxin genes entB and entC1 and the thermonuclease gene nuc.

Authors:  I G Wilson; J E Cooper; A Gilmour
Journal:  Appl Environ Microbiol       Date:  1991-06       Impact factor: 4.792

2.  Inhibition of early response genes prevents changes in global joint metabolomic profiles in mouse post-traumatic osteoarthritis.

Authors:  D R Haudenschild; A K Carlson; D L Zignego; J H N Yik; J K Hilmer; R K June
Journal:  Osteoarthritis Cartilage       Date:  2018-12-18       Impact factor: 6.576
  2 in total

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