Aouatef Ait-Lounis1, Fatima Laraba-Djebari2. 1. USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32 El-Alia, Bab Ezzouar, Algiers, Algeria. 2. USTHB, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, BP 32 El-Alia, Bab Ezzouar, Algiers, Algeria. flaraba@hotmail.com.
Abstract
OBJECTIVE: We previously reported that Androctonus australis hector (Aah) venom and its toxic fraction affect adipose tissue metabolism. However, the contribution of immune system and the role of adipose tissue macrophages (ATMs) in the progression of inflammation induced by scorpion venom remain largely unknown. METHODS: Here we evaluate the capacity of the toxic fraction of Aah venom (FTox-G50) to induce the expression of M1 and M2 markers genes on adipose tissue and isolated stromal vascular cells (SVC). Quantitative real-time PCR was performed on the SVC 24 h after FTox-G50 venom injection to assess the gene expressions of IL12p40, IL23, and other macrophages-associated markers. RESULTS: We found that ATM from FTox-G50-venom-injected mice markedly increased the expressions of IL-12p40 and IL-23. Furthermore, the expression of nitric oxide synthase 2 (an M1 marker) was up-regulated, but the expression of Arginase1 (an M2 marker) was not. Systemic injection of a chemical inhibitor directed against TNF-α binding reduced the expression of inflammatory M1 macrophage markers and the MAPKpk2 gene, a key mediator of inflammatory signaling. CONCLUSION: These results indicate that TNF-α is a physiological regulator of inflammation and macrophage activation induced by scorpion venom.
OBJECTIVE: We previously reported that Androctonus australis hector (Aah) venom and its toxic fraction affect adipose tissue metabolism. However, the contribution of immune system and the role of adipose tissue macrophages (ATMs) in the progression of inflammation induced by scorpion venom remain largely unknown. METHODS: Here we evaluate the capacity of the toxic fraction of Aah venom (FTox-G50) to induce the expression of M1 and M2 markers genes on adipose tissue and isolated stromal vascular cells (SVC). Quantitative real-time PCR was performed on the SVC 24 h after FTox-G50 venom injection to assess the gene expressions of IL12p40, IL23, and other macrophages-associated markers. RESULTS: We found that ATM from FTox-G50-venom-injected mice markedly increased the expressions of IL-12p40 and IL-23. Furthermore, the expression of nitric oxide synthase 2 (an M1 marker) was up-regulated, but the expression of Arginase1 (an M2 marker) was not. Systemic injection of a chemical inhibitor directed against TNF-α binding reduced the expression of inflammatory M1 macrophage markers and the MAPKpk2 gene, a key mediator of inflammatory signaling. CONCLUSION: These results indicate that TNF-α is a physiological regulator of inflammation and macrophage activation induced by scorpion venom.
Authors: Thomas Krausgruber; Katrina Blazek; Tim Smallie; Saba Alzabin; Helen Lockstone; Natasha Sahgal; Tracy Hussell; Marc Feldmann; Irina A Udalova Journal: Nat Immunol Date: 2011-01-16 Impact factor: 25.606
Authors: S Caspar-Bauguil; B Cousin; A Galinier; C Segafredo; M Nibbelink; M André; L Casteilla; L Pénicaud Journal: FEBS Lett Date: 2005-07-04 Impact factor: 4.124
Authors: Amir Jalali; Mohammad H Pipelzadeh; Mohammad Taraz; Ali Khodadadi; Manocher Makvandi; Edward G Rowan Journal: Eur Cytokine Netw Date: 2011-03 Impact factor: 2.737
Authors: Craig R Cohen; Anna-Barbara Moscicki; Mark E Scott; Yifei Ma; Stephen Shiboski; Elizabeth Bukusi; Ibrahim Daud; Anu Rebbapragada; Joelle Brown; Rupert Kaul Journal: AIDS Date: 2010-08-24 Impact factor: 4.177
Authors: Richard J P Smith; Alessandro Faroni; James R Barrow; Jamie Soul; Adam J Reid Journal: Stem Cell Res Ther Date: 2021-03-02 Impact factor: 6.832
Authors: Damien N Barnette; Thomas J Cahill; Mala Gunadasa-Rohling; Carolyn A Carr; Matthew Freeman; Paul R Riley Journal: JCI Insight Date: 2018-02-08