Mohammed Esmail Abdalla Elzaki1, Wanfang Zhang1, Ai Feng1, Xiaoyan Qiou1, Wanxue Zhao1, Zhaojun Han1. 1. Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Jiangsu/The Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing, 210095 Jiangsu, China.
Abstract
BACKGROUND: Imidacloprid is a principal insecticide for controlling rice planthoppers worldwide. Resistance to imidacloprid has been reported in a field population of Laodelphax striatellus. The present work was conducted to study the molecular mechanisms of imidacloprid resistance. RESULTS: An imidacloprid-resistant strain was produced by selecting a field population with imidacloprid for 24 generations. Piperonyl butoxide (PBO) showed a 1.70-fold synergistic effect. Enzyme activity assays were conducted, and cytochrome P450 monooxygenase showed 1.88-fold activity. The mRNA expression levels of 57 P450 genes were compared. Four CYP genes were found to be overexpressed and significantly different to the susceptible strain. Four strains were selected with imidacloprid for a short period, and the expression levels of ten identified detoxification genes were then compared. Only CYP353D1v2 overexpressed and was significantly different to the susceptible strain. Strong correlation was found between CYP353D1v2 expression levels and imidacloprid treatments. Additionally, gene-silencing RNAi via dsRNA feeding showed that depressing the expression of CYP353D1v2 could significantly enhance the sensitivity of L. striatellus to imidacloprid. CONCLUSION: Constitutive overexpression of four CYP genes was associated with imidacloprid resistance in long-term selection, and expression of CYP353D1v2 with imidacloprid resistance in short-term selection in L. striatellus.
BACKGROUND:Imidacloprid is a principal insecticide for controlling rice planthoppers worldwide. Resistance to imidacloprid has been reported in a field population of Laodelphax striatellus. The present work was conducted to study the molecular mechanisms of imidacloprid resistance. RESULTS: An imidacloprid-resistant strain was produced by selecting a field population with imidacloprid for 24 generations. Piperonyl butoxide (PBO) showed a 1.70-fold synergistic effect. Enzyme activity assays were conducted, and cytochrome P450 monooxygenase showed 1.88-fold activity. The mRNA expression levels of 57 P450 genes were compared. Four CYP genes were found to be overexpressed and significantly different to the susceptible strain. Four strains were selected with imidacloprid for a short period, and the expression levels of ten identified detoxification genes were then compared. Only CYP353D1v2 overexpressed and was significantly different to the susceptible strain. Strong correlation was found between CYP353D1v2 expression levels and imidacloprid treatments. Additionally, gene-silencing RNAi via dsRNA feeding showed that depressing the expression of CYP353D1v2 could significantly enhance the sensitivity of L. striatellus to imidacloprid. CONCLUSION: Constitutive overexpression of four CYP genes was associated with imidacloprid resistance in long-term selection, and expression of CYP353D1v2 with imidacloprid resistance in short-term selection in L. striatellus.
Authors: Ying Fang; Jae Young Choi; Dong Hwan Park; Min Gu Park; Jun Young Kim; Minghui Wang; Hyun Ji Kim; Woo Jin Kim; Yeon Ho Je Journal: Plant Pathol J Date: 2020-06-01 Impact factor: 1.795
Authors: Muhammad Hafeez; Muhammad Qasim; Sajjad Ali; Hafiz Kamran Yousaf; Muhammad Waqas; Ehsan Ali; Muhammad Afaq Ahmad; Saad Jan; Muhammad Amjad Bashir; Ali Noman; Mo Wang; Hamed A Gharmh; Khalid Ali Khan Journal: Saudi J Biol Sci Date: 2019-05-22 Impact factor: 4.219