Cintia B Dias1, Lisa G Wood2, Melinda Phang3, Manohar Lal Garg4. 1. Nutraceuticals Research Group, School of Biomedical Sciences and Pharmacy, University of Newcastle, Australia; Hunter Medical Research Institute, University of Newcastle, Australia. Electronic address: cintia.dias@uon.edu.au. 2. Hunter Medical Research Institute, University of Newcastle, Australia; Centre for Asthma and Respiratory Disease, School of Biomedical Sciences and Pharmacy, University of Newcastle, Australia. Electronic address: lisa.wood@newcastle.edu.au. 3. Nutraceuticals Research Group, School of Biomedical Sciences and Pharmacy, University of Newcastle, Australia; Hunter Medical Research Institute, University of Newcastle, Australia. Electronic address: melinda.phang@health.sa.gov.au. 4. Nutraceuticals Research Group, School of Biomedical Sciences and Pharmacy, University of Newcastle, Australia; Hunter Medical Research Institute, University of Newcastle, Australia. Electronic address: manohar.garg@newcastle.edu.au.
Abstract
OBJECTIVE:Absorption of long chain omega-3 polyunsaturated fatty acids (LCn-3PUFA) has been shown to be potentiated when consumed with a high fat meal. However, the effect of different dietary fats on n-3PUFA absorption and postprandial kinetics has not been previously studied. METHOD: In a randomized cross-over design intervention, postprandial incorporation of LCn-3PUFA into plasma lipids following consumption of a meal rich in either saturated fat or omega-6 polyunsaturated fatty acids (n-6PUFA) was investigated. Healthy adult male and female subjects (n=26) were fed an isocaloric meal containing equivalent amount of either butter or sunflower seed oil supplemented with 1.8grams of LCn-3PUFA (300mg eicosapentaenoic acid, 20:5n-3 and 1500mg docosahexaenoic acid, 22:6n-3). RESULTS:Postprandial plasma lipids were enriched with saturated fatty acids and linoleic acid (18:2n-6) following consumption of the butter and the sunflower oil containing meals respectively. The increase in plasma 20:5n-3 and 22:6n-3 levels over the 6hour study period was similar in both the saturated and the n-6 fat groups. CONCLUSION: These results suggest that the expected competition between LCn-3PUFA and n-6PUFA at the absorption level is unlikely; therefore competition at the enzymatic level should be primarily responsible for differences in their metabolic and clinical effects. Trial registered with the Australia New Zealand Trial registry as ACTRN12612000654853.
RCT Entities:
OBJECTIVE: Absorption of long chain omega-3 polyunsaturated fatty acids (LCn-3PUFA) has been shown to be potentiated when consumed with a high fat meal. However, the effect of different dietary fats on n-3PUFA absorption and postprandial kinetics has not been previously studied. METHOD: In a randomized cross-over design intervention, postprandial incorporation of LCn-3PUFA into plasma lipids following consumption of a meal rich in either saturated fat or omega-6 polyunsaturated fatty acids (n-6PUFA) was investigated. Healthy adult male and female subjects (n=26) were fed an isocaloric meal containing equivalent amount of either butter or sunflower seed oil supplemented with 1.8grams of LCn-3PUFA (300mg eicosapentaenoic acid, 20:5n-3 and 1500mg docosahexaenoic acid, 22:6n-3). RESULTS: Postprandial plasma lipids were enriched with saturated fatty acids and linoleic acid (18:2n-6) following consumption of the butter and the sunflower oil containing meals respectively. The increase in plasma 20:5n-3 and 22:6n-3 levels over the 6hour study period was similar in both the saturated and the n-6 fat groups. CONCLUSION: These results suggest that the expected competition between LCn-3PUFA and n-6PUFA at the absorption level is unlikely; therefore competition at the enzymatic level should be primarily responsible for differences in their metabolic and clinical effects. Trial registered with the Australia New Zealand Trial registry as ACTRN12612000654853.