Wei Ben1, Yang Yang1, Jing Yuan1, Jingxia Sun1, Mingli Huang1, Dandan Zhang1, Jianhua Zheng2. 1. Department of Obstetrics and Gynecology, The First Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China. 2. Department of Obstetrics and Gynecology, The First Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China. Electronic address: doctorzheng52@yahoo.com.cn.
Abstract
OBJECTIVE: Human papillomavirus (HPV) infection is a prerequisite of developing cervical cancer, approximately half of which are associated with HPV type 16. There are reports that HPV can disturb the expression pattern of host miRNAs, but its mechanism is not well understood. MATERIALS AND METHODS: In this study, we scanned 11 tumorigenesis related miRNAs in Hela cells that were overexpressed with HPV type 16 E6 protein. RESULTS: We found the expression of miR-21 was upregulated by HPV type 16 E6 protein and meanwhile, the expression of miR-27a and miR-218 was downregulated. Furthermore, we identified that miR-21 overexpression could promote Hela and U2OS cells proliferation by targeting phosphatase-tensin homolog (PTEN), the result of which can be rescued by miR-21 inhibitor. In addition, E6 overexpression could also promote Hela cell migration and invasion. CONCLUSION: Our results indicate that HPV infection and subsequent transformation take place through complex regulatory patterns of gene expression in the host cells, part of which are regulated by the E6 protein.
OBJECTIVE: Human papillomavirus (HPV) infection is a prerequisite of developing cervical cancer, approximately half of which are associated with HPV type 16. There are reports that HPV can disturb the expression pattern of host miRNAs, but its mechanism is not well understood. MATERIALS AND METHODS: In this study, we scanned 11 tumorigenesis related miRNAs in Hela cells that were overexpressed with HPV type 16 E6 protein. RESULTS: We found the expression of miR-21 was upregulated by HPV type 16 E6 protein and meanwhile, the expression of miR-27a and miR-218 was downregulated. Furthermore, we identified that miR-21 overexpression could promote Hela and U2OS cells proliferation by targeting phosphatase-tensin homolog (PTEN), the result of which can be rescued by miR-21 inhibitor. In addition, E6 overexpression could also promote Hela cell migration and invasion. CONCLUSION: Our results indicate that HPV infection and subsequent transformation take place through complex regulatory patterns of gene expression in the host cells, part of which are regulated by the E6 protein.
Authors: Mart Toots; Mart Ustav; Andres Männik; Karl Mumm; Kaido Tämm; Tarmo Tamm; Ene Ustav; Mart Ustav Journal: PLoS Pathog Date: 2017-02-09 Impact factor: 6.823
Authors: Mikhail K Ivanov; Sergei E Titov; Sergei A Glushkov; Victoria V Dzyubenko; Anastasia V Malek; Polina A Arkhangelskaya; Roman B Samsonov; Andrey A Mikhetko; Elena V Bakhidze; Igor V Berlev; Nikolay N Kolesnikov Journal: Oncol Rep Date: 2018-01-12 Impact factor: 3.906
Authors: Elgar Susanne Quabius; Immanuel Merz; Tibor Görögh; Jürgen Hedderich; Jochen Haag; Christoph Röcken; Petra Ambrosch; Markus Hoffmann Journal: Papillomavirus Res Date: 2017-06-16