Kåre Buhlin1,2, Jacob Holmer1, Anders Gustafsson1, Sohvi Hörkkö3, Alan Graham Pockley4, Anders Johansson5, Susanna Paju2, Björn Klinge1,6, Pirkko J Pussinen2. 1. Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden. 2. Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland. 3. Department of Medical Microbiology and Immunology and Medical Research Center, University of Oulu & Nordlab Oulu, Oulu University Hospital, Oulu, Finland. 4. The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK. 5. Department of Molecular Periodontology, Umeå University, Umeå, Sweden. 6. Department of Periodontology, Faculty of Odontology, Malmo University, Malmo, Sweden.
Abstract
AIM: To study antibody responses associated with molecular mimicry in periodontitis. MATERIAL & METHODS: Fifty-four periodontitis cases (mean age 54.0 years) and 44 controls (53.6 years) were examined, after which cases received periodontal treatment. Established immunoassays were used to analyse levels of antibodies against two pathogens, Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg), heat shock proteins (Hsp), Hsp60, Hsp65, and Hsp70, and epitopes of oxidized low-density lipoprotein (oxLDL) (CuOx-LDL and MDA-LDL) in plasma samples that were collected at baseline and after 3 (n = 48) and 6 (n = 30) months. RESULTS: When age, sex, smoking habit, and the number of teeth were considered in multivariate logistic regressions, Aa and Pg IgG, Hsp65-IgA, CuOx-LDL-IgG and -IgM, and MDA-LDL-IgG antibody levels were associated with periodontitis, whereas Hsp60-IgG2 antibody levels were inversely associated. The Aa antibody levels significantly correlated with the levels of IgA antibodies to Hsp65 and Hsp70, and both OxLDL IgA antibody levels. The levels of antibodies to Pg correlated with IgG antibodies to Hsp60, Hsp70, and both oxLDL antibody epitopes. None of the antibody levels changed significantly after treatment. CONCLUSIONS: Periodontitis is associated with persistently high levels of circulating antibodies that are reactive with pathogen- and host-derived antigens.
AIM: To study antibody responses associated with molecular mimicry in periodontitis. MATERIAL & METHODS: Fifty-four periodontitis cases (mean age 54.0 years) and 44 controls (53.6 years) were examined, after which cases received periodontal treatment. Established immunoassays were used to analyse levels of antibodies against two pathogens, Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg), heat shock proteins (Hsp), Hsp60, Hsp65, and Hsp70, and epitopes of oxidized low-density lipoprotein (oxLDL) (CuOx-LDL and MDA-LDL) in plasma samples that were collected at baseline and after 3 (n = 48) and 6 (n = 30) months. RESULTS: When age, sex, smoking habit, and the number of teeth were considered in multivariate logistic regressions, Aa and Pg IgG, Hsp65-IgA, CuOx-LDL-IgG and -IgM, and MDA-LDL-IgG antibody levels were associated with periodontitis, whereas Hsp60-IgG2 antibody levels were inversely associated. The Aa antibody levels significantly correlated with the levels of IgA antibodies to Hsp65 and Hsp70, and both OxLDL IgA antibody levels. The levels of antibodies to Pg correlated with IgG antibodies to Hsp60, Hsp70, and both oxLDL antibody epitopes. None of the antibody levels changed significantly after treatment. CONCLUSIONS:Periodontitis is associated with persistently high levels of circulating antibodies that are reactive with pathogen- and host-derived antigens.
Authors: T Bjarnsholt; K Buhlin; Y F Dufrêne; M Gomelsky; A Moroni; M Ramstedt; K P Rumbaugh; T Schulte; L Sun; B Åkerlund; U Römling Journal: J Intern Med Date: 2018-07-09 Impact factor: 8.989
Authors: Eduardo Gomez-Bañuelos; Linda Johansson; Maximilian F Konig; Anders Lundquist; Merlin Paz; Kåre Buhlin; Anders Johansson; Solbritt Rantapää-Dahlqvist; Felipe Andrade Journal: J Clin Med Date: 2020-06-18 Impact factor: 4.241