Audrey J Gaskins1, Yu-Han Chiu2, Paige L Williams3, Jennifer B Ford4, Thomas L Toth5, Russ Hauser6, Jorge E Chavarro7. 1. Departments of Nutrition, agaskins@hsph.harvard.edu. 2. Departments of Nutrition. 3. Biostatistics, Epidemiology, and. 4. Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA; 5. Vincent Obstetrics and Gynecology, Massachusetts General Hospital and Harvard Medical School, Boston, MA; and. 6. Epidemiology, and Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA; Vincent Obstetrics and Gynecology, Massachusetts General Hospital and Harvard Medical School, Boston, MA; and. 7. Departments of Nutrition, Epidemiology, and Channing Division of Network Medicine, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.
Abstract
BACKGROUND: Preconceptional folate and vitamin B-12 have been linked to beneficial reproductive outcomes in both natural pregnancies and those after assisted reproductive technology (ART) treatment. OBJECTIVE: The objective of the study was to evaluate the associations of serum folate and vitamin B-12 with ART outcomes. DESIGN: This analysis included a random sample of 100 women (154 ART cycles) participating in a prospective cohort study [Environment and Reproductive Health (EARTH)] at the Massachusetts General Hospital Fertility Center (2007-2013). Serum folate and vitamin B-12 were measured in blood samples collected between days 3 and 9 of treatment. Generalized estimating equations with adjustment for age, BMI, and race were used to evaluate the association of serum folate and vitamin B-12 with ART outcomes. RESULTS: Women in the highest quartile of serum folate (>26.3 ng/mL) had 1.62 (95% CI: 0.99, 2.65) times the probability of live birth compared with women in the lowest quartile (<16.6 ng/mL). Women in the highest quartile of serum vitamin B-12 (>701 pg/mL) had 2.04 (95% CI: 1.14, 3.62) times the probability of live birth compared with women in the lowest quartile (<439 pg/mL). Suggestive evidence of an interaction was observed; women with serum folate and vitamin B-12 concentrations greater than the median had 1.92 (95% CI: 1.12, 3.29) times the probability of live birth compared with women with folate and vitamin B-12 concentrations less than or equal to the median. This translated into an adjusted difference in live birth rates of 26% (95% CI: 10%, 48%; P = 0.02). CONCLUSION: Higher serum concentrations of folate and vitamin B-12 before ART treatment were associated with higher live birth rates among a population exposed to folic acid fortification. This trial was registered at clinicaltrials.gov as NCT00011713.
BACKGROUND: Preconceptional folate and vitamin B-12 have been linked to beneficial reproductive outcomes in both natural pregnancies and those after assisted reproductive technology (ART) treatment. OBJECTIVE: The objective of the study was to evaluate the associations of serum folate and vitamin B-12 with ART outcomes. DESIGN: This analysis included a random sample of 100 women (154 ART cycles) participating in a prospective cohort study [Environment and Reproductive Health (EARTH)] at the Massachusetts General Hospital Fertility Center (2007-2013). Serum folate and vitamin B-12 were measured in blood samples collected between days 3 and 9 of treatment. Generalized estimating equations with adjustment for age, BMI, and race were used to evaluate the association of serum folate and vitamin B-12 with ART outcomes. RESULTS:Women in the highest quartile of serum folate (>26.3 ng/mL) had 1.62 (95% CI: 0.99, 2.65) times the probability of live birth compared with women in the lowest quartile (<16.6 ng/mL). Women in the highest quartile of serum vitamin B-12 (>701 pg/mL) had 2.04 (95% CI: 1.14, 3.62) times the probability of live birth compared with women in the lowest quartile (<439 pg/mL). Suggestive evidence of an interaction was observed; women with serum folate and vitamin B-12 concentrations greater than the median had 1.92 (95% CI: 1.12, 3.29) times the probability of live birth compared with women with folate and vitamin B-12 concentrations less than or equal to the median. This translated into an adjusted difference in live birth rates of 26% (95% CI: 10%, 48%; P = 0.02). CONCLUSION: Higher serum concentrations of folate and vitamin B-12 before ART treatment were associated with higher live birth rates among a population exposed to folic acid fortification. This trial was registered at clinicaltrials.gov as NCT00011713.
Authors: Audrey J Gaskins; Janet W Rich-Edwards; Russ Hauser; Paige L Williams; Matthew W Gillman; Elizabeth S Ginsburg; Stacey A Missmer; Jorge E Chavarro Journal: Obstet Gynecol Date: 2014-07 Impact factor: 7.661
Authors: Audrey J Gaskins; Myriam C Afeiche; Diane L Wright; Thomas L Toth; Paige L Williams; Matthew W Gillman; Russ Hauser; Jorge E Chavarro Journal: Obstet Gynecol Date: 2014-10 Impact factor: 7.661
Authors: Keewan Kim; James L Mills; Kara A Michels; Ellen N Chaljub; Jean Wactawski-Wende; Torie C Plowden; Sunni L Mumford Journal: J Acad Nutr Diet Date: 2019-12-23 Impact factor: 4.910
Authors: Mary E Ingle; Michael S Bloom; Patrick J Parsons; Amy J Steuerwald; Pamela Kruger; Victor Y Fujimoto Journal: J Assist Reprod Genet Date: 2016-12-09 Impact factor: 3.412
Authors: Kara A Michels; Jean Wactawski-Wende; James L Mills; Karen C Schliep; Audrey J Gaskins; Edwina H Yeung; Keewan Kim; Torie C Plowden; Lindsey A Sjaarda; Ellen N Chaljub; Sunni L Mumford Journal: Hum Reprod Date: 2017-08-01 Impact factor: 6.918