Literature DB >> 26351695

Biochemical isolation of Argonaute protein complexes by Ago-APP.

Judith Hauptmann1, Daniel Schraivogel1, Astrid Bruckmann1, Sudhir Manickavel2, Leonhard Jakob1, Norbert Eichner1, Janina Pfaff1, Marc Urban3, Stefanie Sprunck3, Markus Hafner2, Thomas Tuschl4, Rainer Deutzmann1, Gunter Meister5.   

Abstract

During microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the TNRC6/GW protein family. Here we used a short GW protein-derived peptide fused to GST and demonstrate that it binds to Ago proteins with high affinity. This allows for the simultaneous isolation of all Ago protein complexes expressed in diverse species to identify associated proteins, small RNAs, or target mRNAs. We refer to our method as "Ago protein Affinity Purification by Peptides" (Ago-APP). Furthermore, expression of this peptide competes for endogenous TNRC6 proteins, leading to global inhibition of miRNA function in mammalian cells.

Entities:  

Keywords:  Argonaute; GW proteins; RNAi; microRNAs; small RNAs

Mesh：

Substances：

Year:  2015        PMID： 26351695      PMCID： PMC4586862          DOI： 10.1073/pnas.1506116112

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  40 in total

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Journal:  Nucleic Acids Res       Date:  2002-01-01       Impact factor: 16.971

2.  Argonaute2 is the catalytic engine of mammalian RNAi.

Authors:  Jidong Liu; Michelle A Carmell; Fabiola V Rivas; Carolyn G Marsden; J Michael Thomson; Ji-Joon Song; Scott M Hammond; Leemor Joshua-Tor; Gregory J Hannon
Journal:  Science       Date:  2004-07-29       Impact factor: 47.728

3.  Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing.

Authors:  Gunter Meister; Markus Landthaler; Yair Dorsett; Thomas Tuschl
Journal:  RNA       Date:  2004-03       Impact factor: 4.942

4.  A biochemical approach to identifying microRNA targets.

Authors:  Fedor V Karginov; Cecilia Conaco; Zhenyu Xuan; Bryan H Schmidt; Joel S Parker; Gail Mandel; Gregory J Hannon
Journal:  Proc Natl Acad Sci U S A       Date:  2007-11-27       Impact factor: 11.205

5.  Studying translational control in Drosophila cell-free systems.

Authors:  Fátima Gebauer; Matthias W Hentze
Journal:  Methods Enzymol       Date:  2007       Impact factor: 1.600

6.  A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain.

Authors:  Susanne Till; Erwan Lejeune; Rolf Thermann; Miriam Bortfeld; Michael Hothorn; Daniel Enderle; Constanze Heinrich; Matthias W Hentze; Andreas G Ladurner
Journal:  Nat Struct Mol Biol       Date:  2007-09-23       Impact factor: 15.369

7.  Isolation of microRNA targets by miRNP immunopurification.

Authors:  George Easow; Aurelio A Teleman; Stephen M Cohen
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8.  Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.

Authors:  Gunter Meister; Markus Landthaler; Agnieszka Patkaniowska; Yair Dorsett; Grace Teng; Thomas Tuschl
Journal:  Mol Cell       Date:  2004-07-23       Impact factor: 17.970

9.  Identification of human microRNA targets from isolated argonaute protein complexes.

Authors:  Michaela Beitzinger; Lasse Peters; Jia Yun Zhu; Elisabeth Kremmer; Gunter Meister
Journal:  RNA Biol       Date:  2007-06-28       Impact factor: 4.652

10.  Reiterated WG/GW motifs form functionally and evolutionarily conserved ARGONAUTE-binding platforms in RNAi-related components.

Authors:  Mahmoud El-Shami; Dominique Pontier; Sylvie Lahmy; Laurence Braun; Claire Picart; Danielle Vega; Mohamed-Ali Hakimi; Steven E Jacobsen; Richard Cooke; Thierry Lagrange
Journal:  Genes Dev       Date:  2007-10-15       Impact factor: 11.361
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  28 in total

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Authors:  Mi Seul Park; Raul Araya-Secchi; James A Brackbill; Hong-Duc Phan; Audrey C Kehling; Ekram W Abd El-Wahab; Daniel M Dayeh; Marcos Sotomayor; Kotaro Nakanishi
Journal:  Mol Cell       Date:  2019-07-16       Impact factor: 17.970

2.  High-Resolution In Vivo Identification of miRNA Targets by Halo-Enhanced Ago2 Pull-Down.

Authors:  Xiaoyi Li; Yuri Pritykin; Carla P Concepcion; Yuheng Lu; Gaspare La Rocca; Minsi Zhang; Bryan King; Peter J Cook; Yu Wah Au; Olesja Popow; Joao A Paulo; Hannah G Otis; Chiara Mastroleo; Paul Ogrodowski; Ryan Schreiner; Kevin M Haigis; Doron Betel; Christina S Leslie; Andrea Ventura
Journal:  Mol Cell       Date:  2020-06-03       Impact factor: 17.970

3.  The biochemical basis for the cooperative action of microRNAs.

Authors:  Daniel Briskin; Peter Y Wang; David P Bartel
Journal:  Proc Natl Acad Sci U S A       Date:  2020-07-13       Impact factor: 11.205

4.  miR-450a Acts as a Tumor Suppressor in Ovarian Cancer by Regulating Energy Metabolism.

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Journal:  Cancer Res       Date:  2019-05-17       Impact factor: 12.701

5.  Phosphorylation of Argonaute proteins affects mRNA binding and is essential for microRNA-guided gene silencing in vivo.

Authors:  Miguel Quévillon Huberdeau; Daniela M Zeitler; Judith Hauptmann; Astrid Bruckmann; Lucile Fressigné; Johannes Danner; Sandra Piquet; Nicholas Strieder; Julia C Engelmann; Guillaume Jannot; Rainer Deutzmann; Martin J Simard; Gunter Meister
Journal:  EMBO J       Date:  2017-06-23       Impact factor: 11.598

6.  Structure-function analysis of microRNA 3'-end trimming by Nibbler.

Authors:  Wei Xie; Ivica Sowemimo; Rippei Hayashi; Juncheng Wang; Thomas R Burkard; Julius Brennecke; Stefan L Ameres; Dinshaw J Patel
Journal:  Proc Natl Acad Sci U S A       Date:  2020-11-16       Impact factor: 11.205

7.  Multivalent Recruitment of Human Argonaute by GW182.

Authors:  Elad Elkayam; Christopher R Faehnle; Marjorie Morales; Jingchuan Sun; Huilin Li; Leemor Joshua-Tor
Journal:  Mol Cell       Date:  2017-08-03       Impact factor: 17.970

Review 8.  AGO unchained: Canonical and non-canonical roles of Argonaute proteins in mammals.

Authors:  Laura Sala; Srividya Chandrasekhar; Joana A Vidigal
Journal:  Front Biosci (Landmark Ed)       Date:  2020-01-01

9.  Investigating the pharmacodynamic durability of GalNAc-siRNA conjugates.

Authors:  Christopher R Brown; Swati Gupta; June Qin; Timothy Racie; Guo He; Scott Lentini; Ryan Malone; Mikyung Yu; Shigeo Matsuda; Svetlana Shulga-Morskaya; Anil V Nair; Christopher S Theile; Karyn Schmidt; Azar Shahraz; Varun Goel; Rubina G Parmar; Ivan Zlatev; Mark K Schlegel; Jayaprakash K Nair; Muthusamy Jayaraman; Muthiah Manoharan; Dennis Brown; Martin A Maier; Vasant Jadhav
Journal:  Nucleic Acids Res       Date:  2020-12-02       Impact factor: 16.971

10.  The Ratio of Toxic-to-Nontoxic miRNAs Predicts Platinum Sensitivity in Ovarian Cancer.

Authors:  Monal Patel; Yinu Wang; Elizabeth T Bartom; Rohin Dhir; Kenneth P Nephew; Daniela Matei; Andrea E Murmann; Ernst Lengyel; Marcus E Peter
Journal:  Cancer Res       Date:  2021-06-15       Impact factor: 12.701
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