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Literature DB >> 26344044

Cas9 gRNA engineering for genome editing, activation and repression.

Samira Kiani^1,2, Alejandro Chavez^3,4,5, Marcelle Tuttle³, Richard N Hall^1,2, Raj Chari⁵, Dmitry Ter-Ovanesyan^3,5, Jason Qian^3,5, Benjamin W Pruitt³, Jacob Beal⁶, Suhani Vora^1,3,5, Joanna Buchthal³, Emma J K Kowal³, Mohammad R Ebrahimkhani^1,7, James J Collins^1,2,3,8,9,10, Ron Weiss^1,2,7, George Church^3,5.

Abstract

We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.

Entities: CellLine Chemical Disease Gene Species

Mesh：

Substances：

Year: 2015 PMID： 26344044 PMCID： PMC4666719 DOI： 10.1038/nmeth.3580

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

25 in total

1. Linear models and empirical bayes methods for assessing differential expression in microarray experiments.

Authors: Gordon K Smyth
Journal: Stat Appl Genet Mol Biol Date: 2004-02-12

2. The Sequence Alignment/Map format and SAMtools.

Authors: Heng Li; Bob Handsaker; Alec Wysoker; Tim Fennell; Jue Ruan; Nils Homer; Gabor Marth; Goncalo Abecasis; Richard Durbin
Journal: Bioinformatics Date: 2009-06-08 Impact factor: 6.937

3. Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells.

Authors: Lior Nissim; Samuel D Perli; Alexandra Fridkin; Pablo Perez-Pinera; Timothy K Lu
Journal: Mol Cell Date: 2014-05-15 Impact factor: 17.970

4. voom: Precision weights unlock linear model analysis tools for RNA-seq read counts.

Authors: Charity W Law; Yunshun Chen; Wei Shi; Gordon K Smyth
Journal: Genome Biol Date: 2014-02-03 Impact factor: 13.583

Review 5. Bridging the gap: a roadmap to breaking the biological design barrier.

Authors: Jacob Beal
Journal: Front Bioeng Biotechnol Date: 2015-01-20

6. Inducible in vivo genome editing with CRISPR-Cas9.

Authors: Lukas E Dow; Jonathan Fisher; Kevin P O'Rourke; Ashlesha Muley; Edward R Kastenhuber; Geulah Livshits; Darjus F Tschaharganeh; Nicholas D Socci; Scott W Lowe
Journal: Nat Biotechnol Date: 2015-02-18 Impact factor: 54.908

7. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system.

Authors: Baohui Chen; Luke A Gilbert; Beth A Cimini; Joerg Schnitzbauer; Wei Zhang; Gene-Wei Li; Jason Park; Elizabeth H Blackburn; Jonathan S Weissman; Lei S Qi; Bo Huang
Journal: Cell Date: 2013-12-19 Impact factor: 41.582

8. RNA-guided editing of bacterial genomes using CRISPR-Cas systems.

Authors: Wenyan Jiang; David Bikard; David Cox; Feng Zhang; Luciano A Marraffini
Journal: Nat Biotechnol Date: 2013-01-29 Impact factor: 54.908

9. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs.

Authors: Yanfang Fu; Jeffry D Sander; Deepak Reyon; Vincent M Cascio; J Keith Joung
Journal: Nat Biotechnol Date: 2014-01-26 Impact factor: 54.908

10. RNA-guided gene activation by CRISPR-Cas9-based transcription factors.

Authors: Pablo Perez-Pinera; D Dewran Kocak; Christopher M Vockley; Andrew F Adler; Ami M Kabadi; Lauren R Polstein; Pratiksha I Thakore; Katherine A Glass; David G Ousterout; Kam W Leong; Farshid Guilak; Gregory E Crawford; Timothy E Reddy; Charles A Gersbach
Journal: Nat Methods Date: 2013-07-25 Impact factor: 28.547

107 in total

Cas9 gRNA engineering for genome editing, activation and repression.

1. Linear models and empirical bayes methods for assessing differential expression in microarray experiments.

2. The Sequence Alignment/Map format and SAMtools.

3. Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells.

4. voom: Precision weights unlock linear model analysis tools for RNA-seq read counts.

Review 5. Bridging the gap: a roadmap to breaking the biological design barrier.

6. Inducible in vivo genome editing with CRISPR-Cas9.

7. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system.

8. RNA-guided editing of bacterial genomes using CRISPR-Cas systems.

9. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs.

10. RNA-guided gene activation by CRISPR-Cas9-based transcription factors.

Review 1. CRISPR applications in ophthalmologic genome surgery.

2. Genome Editing Reveals Glioblastoma Addiction to MicroRNA-10b.

Review 3. CRISPR Tools for Systematic Studies of RNA Regulation.

Review 4. Neuronal differentiation strategies: insights from single-cell sequencing and machine learning.

Review 5. CRISPR-Cas type II-based Synthetic Biology applications in eukaryotic cells.

Review 6. Genome-Editing Technologies: Principles and Applications.

7. Complex transcriptional modulation with orthogonal and inducible dCas9 regulators.

Review 8. What rheumatologists need to know about CRISPR/Cas9.

Review 9. Attenuation of Inherited and Acquired Retinal Degeneration Progression with Gene-based Techniques.

10. CRISPR Technology for Breast Cancer: Diagnostics, Modeling, and Therapy.